PDF DatasheetAntibody (Suitable for clinical applications)
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:25-1:50 |
| Pretreatment | No Pretreatment Required |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Pancreatic Polypeptide Information for Pathologists
Summary:
Pancreatic polypeptide (PP), discovered in 1968 (Endocrinology 1968;83:1323) is a 36 amino acid peptide (molecular weight 4,200 Da) produced and secreted by PP cells (originally termed F cells) of the pancreas. PP cells are primarily located in the Islets of Langerhans and represent about 10% of the islet cells (Virchows Arch A Pathol Anat Histol 1980;387:319). Pathophysiology PP is synthesized as the amino portion of a larger 95 amino acid precursor. The biological activity is located in the carboxyl hexapeptide and requires the C terminal amide.
Common Uses By Pathologists:
PP cells can be identified in pancreatic polypeptide cell hyperplasia (PPCH), PPomas (tumors composed exclusively or mostly of pancreatic polypeptide cells) and mixed pancreatic neuroendocrine tumor. Case reports 32 year old man with malignant pancreatic polypeptide secreting tumour of islet cells (HPB Surg 1993;6:301307). 37 year old woman with pancreatic polypeptide hyperplasia causing watery diarrhea syndrome (Can J Surg 1999;42:55). 46 year old woman with symptomatic pancreatic polypeptide-secreting tumor of the distal pancreas (Int J Gastrointest Cancer 2002;32:153).
| Pancreatic Polypeptide General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 10.4 kDa | |
| Chromosomal Location | |
| q21.31 [chr: 17] [chr_start: 43940804] [chr_end: 43942468] [strand: -1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PPY |
| Entrez Gene ID | 5539 |
| RefSeq Protein Accession(s) | NP_002713 |
| RefSeq mRNA Accession(s) | XM_011524978; NM_001319209; NM_002722 |
| RefSeq Genomic Accession(s) | NC_000017 |
| UniProt ID(s) | P01298 |
| PharmGKB ID(s) | PA33690 |
| KEGG Gene ID(s) | hsa:5539 |
| General Description of Pancreatic Polypeptide . | |
| This antibody reacts with pancreatic polypeptide. It stains the periphery of islets, exocrine pancreatic parenchyma and the epithelium of small and medium sized ducts and acinar cells. | |
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