PDF DatasheetAntibody (Suitable for clinical applications)
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | Only Available as Predilute |
| Pretreatment | EDTA Buffer pH 8.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Pax-8 Information for Pathologists
Summary:
Also called Paired box 8. Discovered in 1990 (Development 1990;110:643). Paired domains are composed of two helix-turn-helix subdomains. The N-terminal subdomain and the C-terminal subdomain, both of which can bind DNA independently (Cell 1995;80:639). Structurally similar to PAX2 and PAX5.
Common Uses By Pathologists:
Differentiate PAX8+ primary or metastatic tumors (kidney, Müllerian [but not mucinous, Am J Surg Pathol 2011;35:1837], thymic, thyroid) from PAX8- tumors (adrenal, breast, GI [most], lung, prostate, Am J Surg Pathol 2011;35:816). Differentiate prostatic mesonephric remnant hyperplasia (PAX8+) from prostatic adenocarcinoma (PAX8-, Am J Surg Pathol 2011;35:1054). Differentiate metastatic clear cell renal cell carcinoma (PAX2+ or PAX8+ / inhibinA neg) from hemangioblastoma (PAX2-, PAX8-, inhibin A+, Am J Surg Pathol 2011;35:262). Differentiate endosalpingiosis in axillary lymph nodes (PAX8+ / WT1+) from metastatic breast carcinoma (PAX8-, WT1-, Am J Surg Pathol 2010;34:1211). Differentiate renal collecting duct carcinoma (PAX8+ / p63-) from urothelial carcinoma of upper urinary tract (PAX8- / p63+, Am J Surg Pathol 2010;34:965).
| Pax-8 General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 48.2 kDa | |
| Chromosomal Location | |
| q14.1 [chr: 2] [chr_start: 113215997] [chr_end: 113278921] [strand: -1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PAX8 |
| Entrez Gene ID | 7849 |
| RefSeq Protein Accession(s) | NP_039247; NP_039246; NP_054698; NP_003457 |
| RefSeq mRNA Accession(s) | NM_013951; NM_013992; NM_013953; NM_003466; NM_013952 |
| RefSeq Genomic Accession(s) | NG_012384; NC_000002 |
| UniProt ID(s) | Q06710 |
| PharmGKB ID(s) | PA32962 |
| KEGG Gene ID(s) | hsa:7849 |
| Associated Diseases (KEGG IDs) | Hypothyroidism, congenital, non-goitrous, 2 (CHNG2) [MIM:218700]: A disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue. {ECO:0000269|PubMed:11232006, ECO:0000269|PubMed:11502839, ECO:0000269|PubMed:9590296}. The disease is caused by mutations affecting the gene represented in this entry. |
| General Description of Pax-8 . | |
| PAX 8 is expressed in a high percentage of renal cell carcinomas and ovarian cancers. This mouse monoclonal PAX 8 antibody [4H7B3] has been designed to target restricted epitopes, and exhibits higher specificity and provides sharper staining than the PAX 8 rabbit polyclonal antibody. Unlike the polyclonal PAX 8, this mouse monoclonal antibody does not stain B-cells, and does not recognize epitopes of pancreatic origin and neuroendocrine cells in stomach and colon; thus providing superior specificity. The expression of the mouse monoclonal PAX 8 targets antigens found in normal kidney, thyroid and cervix, but not normal ovary. PAX 8 stains nuclei exclusively and performs well in formalin-fixed paraffin-embedded tissues. | |
There are no reviews yet.