PDF DatasheetAntibody (Suitable for clinical applications)
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:10-1:20 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Perforin Information for Pathologists
Summary:
Perforin is the pore-forming protein that drives osmotic lysis of target cells enables granzymes to enter the target cells and activate apoptosis. The expression of perforin is primarily upregulated in activated cytotoxic cells such as CD8+ T-cells and NK cells but is also found in some populations of CD4+ T-cells.
| Perforin General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 61.4 kDa | |
| Chromosomal Location | |
| q22.1 [chr: 10] [chr_start: 70597348] [chr_end: 70602759] [strand: -1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PRF1 |
| Entrez Gene ID | 5551 |
| RefSeq Protein Accession(s) | NP_001076585; NP_005032 |
| RefSeq mRNA Accession(s) | NM_001083116; NM_005041; |
| RefSeq Genomic Accession(s) | NG_009615; NC_000010 |
| UniProt ID(s) | P14222 |
| PharmGKB ID(s) | PA33732 |
| KEGG Gene ID(s) | hsa:5551 |
| Associated Diseases (KEGG IDs) | Familial hemophagocytic lymphohistiocytosis 2 (FHL2) [MIM:603553]: A rare disorder characterized by immune dysregulation with hypercytokinemia, defective function of natural killer cell, and massive infiltration of several organs by activated lymphocytes and macrophages. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, and less frequently neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits and ataxia. {ECO:0000269|PubMed:10583959, ECO:0000269|PubMed:11179007}. The disease is caused by mutations affecting the gene represented in this entry. |
| General Description of Perforin . | |
| This antibody is specific to a 70 kDa protein, which is a potent cytolytic pore-forming protein. Perforin is a specific marker of functionally active cytotoxic T-lymphocytes and natural killer cells. Perforin mediates cytolysis of target cells by membrane damage and apoptosis. | |
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