PDF DatasheetAll,species Anti-Phosphotyrosine Antibody Product Attributes
Species: All,species
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunoprecipitation (1-2ug of antibody/500ug protein Lysate), Immunohistochemistry (IHC) (Formalin-fixed) (1-2ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Phosphotyrosine Antibody Clone: PY20
Clone PY20 Host and Isotype: Mouse IgG2b
Anti-All,species Phosphotyrosine Positive Control Sample: MCF-7, MDA-231, T47-D cells or breast carcinoma
Cellular Localization of Antibody Depends upon the location of phosphorylated target
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Phosphotyrosine conjugated to KLH
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Phosphotyrosine Previously Observed Antibody Staining Patterns
Limitations and Warranty
enQuire Bio’s Phosphotyrosine Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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