PDF DatasheetAntibody (Suitable for clinical applications)
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:25-1:50 |
| Pretreatment | EDTA Buffer pH 8.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Podoplanin Information for Pathologists
Summary:
38 kDa membrane mucoprotein originally detected on surface of rat podocytes. Antibody D2-40 reacts to an O-linked, 40 kDa sialoglycoprotein found on lymphatic endothelium, fetal testis and on the surface of testicular germ cell tumors (also other cells as indicated below). Uses by pathologists Identify lymphatic endothelium to determine presence of lymphatics in various conditions, as well as origin of vascular tumors (J Histochem Cytochem 2009;57:643). Identify lymphatic endothelium to determine lymphatic invasion (Ann Diagn Pathol 2009;13:168).
Common Uses By Pathologists:
Identify lymphatic endothelium to determine presence of lymphatics in various conditions, as well as origin of vascular tumors (J Histochem Cytochem 2009;57:643). Identify lymphatic endothelium to determine lymphatic invasion (Ann Diagn Pathol 2009;13:168). Differentiate mesothelioma (positive, even in effusions, Diagn Cytopathol 2007;35:342) from adenocarcinoma (negative, Cancer 2007;109:933). Note: prognostic significance of lymphatic invasion using D2-40 versus H&E only is unclear:. Not clearly significant – breast carcinoma (Mod Pathol 2009;22:216).
| Podoplanin General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 16.7 kDa | |
| Chromosomal Location | |
| p36.21 [chr: 1] [chr_start: 13583465] [chr_end: 13617957] [strand: 1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PDPN |
| Entrez Gene ID | 10630 |
| RefSeq Protein Accession(s) | NP_938203; NP_001006626; XP_006710358; NP_006465; NP_001006625 |
| RefSeq mRNA Accession(s) | XM_006710295; NM_001006624; XM_024451415; XM_024451404; NM_198389; NM_006474; NM_001006625 |
| RefSeq Genomic Accession(s) | NC_000001 |
| UniProt ID(s) | Q86YL7 |
| PharmGKB ID(s) | PA142671187 |
| KEGG Gene ID(s) | hsa:10630 |
| General Description of Podoplanin . | |
| Podoplanin is a 38kDa membrane mucoprotein originally detected on the surface of rat podocytes .The antibody against podoplanin (D2-40) reacts to a 40 kDA sialogylcoprotein found on lymphatic endothelium, fetal testis, and on the surface of testicular germ cell tumors. (1) | |
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