PDF DatasheetAntibody (Suitable for clinical applications)
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | HistoZyme |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
S-100 Information for Pathologists
Summary:
Acidic protein, 100% Soluble in ammonium sulfate at neutral pH (derivation of name). S100 protein family is multigenic group of nonubiquitous cytoplasmic EF-hand Ca2+-binding proteins, sharing significant structural similarities at both genomic and protein levels; S100 protein family has 24 known human members each coded by a separate gene; at least 19 on chromosome 1q21 (Hepat Mon 2012;12:e7240). Helps regulate cell contraction, motility, growth, differentiation, cell cycle progression, transcription and secretion. Structurally similar to calmodulin. Interpretation
Common Uses By Pathologists:
Common marker of neural tissue / lesions and melanoma. Differentiate plexiform schwannoma (S100+, Am J Surg Pathol 2005;29:1042) from MPNST (weak / negative / patchy). MelanA and MART1 appear to be superior to S100 for evaluating sentinel lymph nodes for melanoma (Am J Surg Pathol 2001;25:1039). Microscopic (histologic) images Images hosted on PathOut server:.
| S-100 General Information | |
|---|---|
| Alternate Names | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Multiple |
| General Description of S-100 . | |
| This antibody stains with S-100 protein. S-100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S-100a and S-100b proteins are two members of the S-100 family. S-100a is composed of an alpha and beta chain whereas S-100b is composed of two beta chains. This antibody stains schwannomas, ependymomas, astrogliomas, almost all benign and malignant melanomas and their metastases. | |
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