Immunohistochemical staining of S-100 Protein of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

S-100 Protein [Polyclonal] Antibody (cGMP).

$ 158.00 – $ 428.00

SKU: QIVD187
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Rabbit Undetermined

SKU: QIVD187-0.1ml Categories: , , , , , , , , , Tag:
 PDF Datasheet

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes

Specification Recommendation
Recommended Dilution (Conc) 1:50-1:200
Pretreatment HistoZyme
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Polyclonal
Anti-S-100 Protein Antibody Clone: Polyclonal
Host and Isotype: Rabbit Undetermined
Recommended Positive Control Sample: Melanoma
Cellular Localization of Antibody Polyclonal Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Affinity purified S-100 protein isolated from cow brain.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

S-100 Protein Information for Pathologists

Summary:

95 amino acid calcium-binding protein first purified from placenta in 1992 (Eur J Biochem 1992;207:541); 95 amino acid calcium-binding protein; may initiate carcinogenesis in some epithelial tumors. Caution: S100 (the neural / melanocytic marker) is also described as S100p or S100P in the literature. Pathophysiology Intracellular S100P interacts with ezrin, and extracellular S100P activates the receptor for advanced glycation endproducts. Associated with aggressive disease in peripheral intrahepatic cholangiocarcinoma (Am J Surg Pathol 2011;35:590).

Common Uses By Pathologists:

Diagnose pancreatic adenocarcinoma in FNA specimens (Am J Clin Pathol 2008;129:81, Diagn Cytopathol 2011 Apr 28 [Epub ahead of print]). Differentiate bile duct adenocarcinoma from reactive lesions at biopsy, as part of a immunohistochemical panel with pVHL and IMP3 (Hum Pathol 2010;41:1210). Differentiate urothelial carcinoma (S100P+ in 78-86%) from prostatic adenocarcinoma (S100P-). Possible biological indicator (due to downregulation) of cadmium toxicity in placentas (Sci Total Environ 2011;410:53). Microscopic (histologic) images

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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