.jpg "S100A9 IHC human Tonsil (S100A9 1011)")
PDF DatasheetHuman Anti-S100A9 Antibody Product Attributes
S100A9 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-S100A9 antibody staining is expected to be primarily localized to the cell junctions and cytosol.
Observed Antibody Staining Data By Tissue Type:
Variations in S100A9 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in hematopoietic cells in the bone marrow, squamous epithelial cells in the cervix, uterine, esophagus and oral mucosa, epidermal cells in the skin, cells in the red pulp in spleen and squamous epithelial cells in the tonsil and vagina. More moderate antibody staining intensity was present in hematopoietic cells in the bone marrow, squamous epithelial cells in the cervix, uterine, esophagus and oral mucosa, epidermal cells in the skin, cells in the red pulp in spleen and squamous epithelial cells in the tonsil and vagina. Low, but measureable presence of S100A9 could be seen in cells in the white pulp in spleen. We were unable to detect S100A9 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of S100A9 expression as measured by anti-S100A9 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | – | – | + | – | + | – | + | – | ++ | – | – | – | – | – | – | ++ | – | – | – | – |
| S100A9 Variability | ++ | + | ++ | + | ++ | + | ++ | ++ | ++ | ++ | + | ++ | ++ | ++ | + | ++ | + | + | + | ++ |
| S100A9 General Information | |
|---|---|
| Alternate Names | |
| S100 calcium-binding protein A9, S100A9, migration inhibitory factor-related protein 14, MRP14, calgranulin B, S100A9 | |
| Molecular Weight | |
| 14kDa | |
| Chromosomal Location | |
| 1q21 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | S100A9 |
| Entrez Gene ID | 6280 |
| Ensemble Gene ID | ENSG00000163220 |
| RefSeq Protein Accession(s) | NP_002956 |
| RefSeq mRNA Accession(s) | NM_002965 |
| RefSeq Genomic Accession(s) | NC_018912, NC_000001 |
| UniProt ID(s) | P06702 |
| UniGene ID(s) | P06702 |
| HGNC ID(s) | 10499 |
| Cosmic ID(s) | S100A9 |
| KEGG Gene ID(s) | hsa:6280 |
| PharmGKB ID(s) | PA34911 |
| General Description of S100A9. | |
| Recognizes a 14kDa protein, identified as S100A9 (also known as Calgranulin B or MRP-14); expressed by granulocytes, monocytes, by tissue macrophages.The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm,/or nucleus of a wide range of cells,, involved in the regulation of a number of cellular processes such as cell cycle progression, differentiation. Altered expression of this protein is associated with the disease cystic fibrosis. This MAb reacts with neutrophils, monocytes, macrophages,, has been shown as an important marker for identifying macrophages in tissue sections. Among cells that are now recognized as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells,, Langerhans cells (in normal tissues), epithelioid cells, Langerhans-type, foreign-body-type multinucleated giant cells (in inflamed tissues). | |
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