PDF DatasheetAntibody (Suitable for clinical applications)
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | EDTA Buffer pH 8.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
SOX-10 Information for Pathologists
Summary:
SRY-related HMG-box 10 (SOX10) protein. Transcription factor known to be crucial in the specification of the neural crest and maintenance of Schwann cells and melanocytes. Expressed in nuclei of melanocytes, breast myoepithelial cells. Clinical features Usually positive nuclear staining in breast basal-like / unclassified triple negative / metaplastic carcinoma (Hum Pathol 2013;44:959).
Notable Clinical Features:
Usually positive nuclear staining in breast basal-like / unclassified triple negative / metaplastic carcinoma (Hum Pathol 2013;44:959). Usually negative / rarely positive staining in breast luminal A/B or HER2+ carcinoma (5% positive), ER-/PR- DCIS (4% positive), phyllodes tumor, fibroadenoma. Specific (93%), but not very sensitive (67%) for malignant peripheral nerve sheath tumor over synovial sarcoma (Mod Pathol 2014;27:55). Must interpret with caution in intraneural tumors. In major salivary glands, expressed in nuclei of acini and both luminal and abluminal cells of intercalated ducts, but not in other sites (Mod Pathol 2013;26:1041).
Common Uses By Pathologists:
Melanoma marker (100%, Appl Immunohistochem Mol Morphol 2014;22:142). Nevi are positive (Nat Cell Biol 2012;14:882), desmoplastic melanoma may be only focally positive (Appl Immunohistochem Mol Morphol 2013;21:506). SOX10 shows an increased specificity for soft tissue tumors of neural crest origin compared with S100 (Appl Immunohistochem Mol Morphol 2012;20:445). MITF1 and SOX10 can be used to differentiate melanoma in situ from actinic keratosis with melanocytic hyperplasia (Am J Dermatopathol 2014;36:124). Microscopic (histologic) images
| SOX-10 General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 49.9 kDa | |
| Chromosomal Location | |
| q13.1 [chr: 22] [chr_start: 37970686] [chr_end: 37987422] [strand: -1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | SOX10 |
| Entrez Gene ID | 6663 |
| RefSeq Protein Accession(s) | NP_008872 |
| RefSeq mRNA Accession(s) | ; NM_006941 |
| RefSeq Genomic Accession(s) | NG_007948; NC_000022 |
| UniProt ID(s) | P56693 |
| PharmGKB ID(s) | PA36027 |
| KEGG Gene ID(s) | hsa:6663 |
| Associated Diseases (KEGG IDs) | Waardenburg syndrome 2E (WS2E) [MIM:611584]: An autosomal dominant auditory-pigmentary disorder characterized by sensorineural deafness, pigmentary disturbances of the hair, skin and eyes, and absence of dystopia canthorum which is the lateral displacement of the inner canthus of each eye. Individuals with WS2E may have neurologic abnormalities, including mental impairment, myelination defects, and ataxia. Some patients can manifest features of Kallmann syndrome. {ECO:0000269|PubMed:10441344, ECO:0000269|PubMed:17999358, ECO:0000269|PubMed:21898658}. The disease is caused by mutations affecting the gene represented in this entry.; Waardenburg syndrome 4C (WS4C) [MIM:613266]: A disorder characterized by the association of Waardenburg features (depigmentation and deafness) with the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease). {ECO:0000269|PubMed:18348274, ECO:0000269|PubMed:21898658, ECO:0000269|PubMed:9462749}. The disease is caused by mutations affecting the gene represented in this entry.; Peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome and Hirschsprung disease (PCWH) [MIM:609136]: A complex neurocristopathy that includes features of 4 distinct syndromes: peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome and Hirschsprung disease. {ECO:0000269|PubMed:10762540, ECO:0000269|PubMed:15004559, ECO:0000269|PubMed:19208381, ECO:0000269|PubMed:21898658}. The disease is caused by mutations affecting the gene represented in this entry. |
| General Description of SOX-10 . | |
| SOX-10, a member of the sex-determining region Y-related HMG-box family, is a transcription factor that is expressed in pre-migratory and migrating neural crest cells. Following migration it remains expressed in cells destined for glial, schwannian, and melanocytic differentiation. SOX10 is widely expressed in normal human tissues including melanocytes and breast tissue. SOX10 is also an important marker in malignant tumors such as melanoma, and breast carcinomas of basal-like and triple negative type, gliomas, and benign tumors such as schwannomas. More importantly, SOX10 has been shown to be expressed in 97-100% of desmoplastic and spindle cell melanomas and has also been shown to be expressed in 100% of nevi. Spindle cell and desmoplastic melanomas are rare variants of invasive cutaneous melanoma, with an annual incidence rate of approximately 2 per 100,000. The majority of oligodendrogliomas and a large percentage of astrocytomas and poorly differentiated glioblastomas have also been shown to express SOX10. | |
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