PDF DatasheetHuman and Broad Species Reactivity Anti-SUMO-2/3 Antibody Product Attributes
Species: Human and Broad Species Reactivity
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-SUMO-2/3 Antibody Clone: SM23/496
Clone SM23/496 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Broad Species Reactivity SUMO-2/3 Positive Control Sample: HeLa cells or breast carcinoma
Cellular Localization of Antibody Nuclear (SUMO-2); Cytoplasmic (SUMO-3)
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Mouse / IgG1, kappa
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
SUMO-2/3 Previously Observed Antibody Staining Patterns
| SUMO-2/3 General Information | |
|---|---|
| Alternate Names | |
| Recombinant human SUMO2 protein, anti-Recombinant human SUMO2 protein antibody | |
| Molecular Weight | |
| 11-13kDa | |
| Chromosomal Location | |
| 17q25.1 (SUMO-2) & 21q22.3 (SUMO-3) | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | SUMO2 & SUMO3 |
| Entrez Gene ID | 6613 |
| Ensemble Gene ID | ENSG00000188612 |
| RefSeq Protein Accession(s) | NP_001005849, NP_008868 |
| RefSeq mRNA Accession(s) | NM_001005849, NM_006937 |
| RefSeq Genomic Accession(s) | NC_000017, NC_018928 |
| UniProt ID(s) | P55855, A0A024R8S3, P61956 |
| UniGene ID(s) | P55855, A0A024R8S3, P61956 |
| HGNC ID(s) | 11125 |
| Cosmic ID(s) | SUMO2 |
| KEGG Gene ID(s) | hsa:6613 |
| PharmGKB ID(s) | PA134914683 |
| General Description of SUMO-2/3. | |
| This MAb reacts with both SUMO-2, SUMO-3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, 2, 3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, E3 cascade enzymes for conjugation. However, SUMO, ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis, protein stability. The unconjugated SUMO-1, 2, 3 proteins localize to the nuclear membrane, nuclear bodies, cytoplasm, respectively. SUMO-1 utilizes Ubc9 for conjugation to several target proteins, which include MDM2, p53, PML, RanGap1. SUMO-2, 3 contribute to a greater percentage of protein modification than does SUMO-1, unlike SUMO-1, they can form polymeric chains. In addition, SUMO-3 regulates beta-Amyloid generation, may be critical in the onset or progression of Alzheimers disease. | |
Limitations and Warranty
enQuire Bio’s SUMO-2/3 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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