PDF DatasheetAntibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:250-1:500 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Polyclonal
Anti-Survivin Antibody Clone: Polyclonal
Host and Isotype: Rabbit Undetermined
Recommended Positive Control Sample: Stomach
Cellular Localization of Antibody Polyclonal Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: A synthetic peptide derived from the C-terminal region of survivin.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
Survivin Information for Pathologists
Summary:
Inhibitor of apoptosis protein; may also enhance proliferative activity of tumor cells. Overexpression enhances cell survival. Interpretation Cytoplasmic staining. Positive staining – normal
| Survivin General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 16.4 kDa | |
| Chromosomal Location | |
| q25.3 [chr: 17] [chr_start: 78214186] [chr_end: 78225636] [strand: 1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | BIRC5 |
| Entrez Gene ID | 332 |
| RefSeq Protein Accession(s) | NP_001012270; NP_001159; NP_001012271 |
| RefSeq mRNA Accession(s) | NM_001012270; NM_001012271; XR_243654; XR_934452; NM_001168 |
| RefSeq Genomic Accession(s) | NG_029069; NC_000017 |
| UniProt ID(s) | O15392 |
| PharmGKB ID(s) | PA25362 |
| KEGG Gene ID(s) | hsa:332 |
| General Description of Survivin . | |
| This antibody reacts with a 16.5 kDa protein, known as survivin. Survivin is an inhibitor of apoptosis proteins. It is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle. Disruption of survivin-microtubule interactions results in loss of survivin’s anti-apoptotic function and increased caspase-3 activity. | |
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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