.jpg "TAG-72 CA72.4 IHC human Colon Carcinoma (SPM536)")
PDF DatasheetHuman, Cow, Canine, and Rat Anti-TAG-72 / CA72.4 Antibody Product Attributes
Species: Human, Cow, Canine, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-TAG-72 / CA72.4 Antibody Clone: SPM536
Clone SPM536 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Cow, Canine, and Rat TAG-72 / CA72.4 Positive Control Sample: Jurkat cells, Breast or lung carcinoma.
Cellular Localization of Antibody Cytoplasmic, cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Purified TAG-72 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
TAG-72 / CA72.4 Previously Observed Antibody Staining Patterns
| TAG-72 / CA72.4 General Information | |
|---|---|
| Alternate Names | |
| CA 72.4, Tumor associated glycoprotein 72, anti-CA 72.4 antibody, anti-Tumor associated glycoprotein 72 antibody | |
| Molecular Weight | |
| 220kDa | |
| Chromosomal Location | |
| Not Known | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Not Known |
| Entrez Gene ID | 182875 |
| Ensemble Gene ID | WBGene00006447 |
| RefSeq Protein Accession(s) | NP_492674 |
| RefSeq mRNA Accession(s) | NM_060273 |
| RefSeq Genomic Accession(s) | NC_003279 |
| UniProt ID(s) | Q9XVS1 |
| UniGene ID(s) | Q9XVS1 |
| Cosmic ID(s) | tag-72 |
| KEGG Gene ID(s) | cel:CELE_C25A1.3 |
| General Description of TAG-72 / CA72.4. | |
| Recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity, 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma, adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72, anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma. | |
Limitations and Warranty
enQuire Bio’s TAG-72 / CA72.4 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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