Immunohistochemical staining of Thrombospondin of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Thrombospondin [A6.1] Antibody (cGMP).

$ 135.00 – $ 775.00

SKU: QIVD216
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG1

SKU: QIVD216-0.1ml Categories: , , , , , , , , , Tag:
 PDF Datasheet

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes

Specification Recommendation
Recommended Dilution (Conc) 1:25-1:50
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at RoomTemperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Thrombospondin Antibody Clone: A6.1
Host and Isotype: Mouse IgG1
Recommended Positive Control Sample: Tonsil
Cellular Localization of Antibody A6.1 Staining: Secretory granules, Golgi complex, endoplasmic reticulum
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Thrombospondin Information for Pathologists

Summary:

Thrombospondins are a family of secreted glycoproteins with that negatively regulate angiogenesis. Thrombospondin is produced by immature astrocytes during brain development, which promotes the development of new synapses and may be a useful marker for platelet activation and aggregation.

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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