PDF DatasheetAntibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:10-1:25 |
| Pretreatment | EDTA Buffer pH 8.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Monoclonal
Anti-Thyroid Peroxidase Antibody Clone: MoAb47
Host and Isotype: Mouse IgG1
Recommended Positive Control Sample: Thyroid
Cellular Localization of Antibody MoAb47 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Human thyroid peroxidase (hTPO) purified from thyroid microsomes by immunoaffinity chromatography.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
Thyroid Peroxidase Information for Pathologists
Summary:
Thyroid peroxidase (TPO) is an enzyme involved in TH synthesis. It is expressed on the apical surface of thyroid folliular cells.
Common Uses By Pathologists:
Malignant thyroid tumors exhibit an anomaly in TPO resulting in lower affinity for anti-TPO. TPO staining may help differentiate between benign and malignant thyroid tumors. TPO staining is also useful for classification of solitary cold thyroid nodules in FNA cytology samples.
| Thyroid Peroxidase General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 103 kDa | |
| Chromosomal Location | |
| p25.3 [chr: 2] [chr_start: 1374066] [chr_end: 1543711] [strand: 1]; [chr: CHR_HSCHR2_4_CTG1] [chr_start: 1372594] [chr_end: 1540021] [strand: 1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | TPO |
| Entrez Gene ID | 7173 |
| RefSeq Protein Accession(s) | NP_783652; NP_001193674; XP_011508683; NP_001193673; NP_783650; NP_000538; NP_783653 |
| RefSeq mRNA Accession(s) | XM_011510380; XM_024453091; NM_001206744; XM_024453085; XM_024453086; XM_024453087; NM_000547; XM_024453090; NM_001206745; NM_175721; XM_024453088; XM_024453092; NM_175719; NM_175720; XM_024453089; XM_024453093; NM_175722 |
| RefSeq Genomic Accession(s) | ; NC_000002; NT_187529; NG_011581 |
| UniProt ID(s) | P07202 |
| PharmGKB ID(s) | PA36694 |
| KEGG Gene ID(s) | hsa:7173 |
| Associated Diseases (KEGG IDs) | An alternative splicing in the thyroperoxidase mRNA can cause Graves’ disease.; Thyroid dyshormonogenesis 2A (TDH2A) [MIM:274500]: A disorder due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete. {ECO:0000269|PubMed:10084596, ECO:0000269|PubMed:10468986, ECO:0000269|PubMed:11061528, ECO:0000269|PubMed:11415848, ECO:0000269|PubMed:11874711, ECO:0000269|PubMed:11916616, ECO:0000269|PubMed:12213873, ECO:0000269|PubMed:12490071, ECO:0000269|PubMed:12843174, ECO:0000269|PubMed:12864797, ECO:0000269|PubMed:12938097, ECO:0000269|PubMed:16284446, ECO:0000269|PubMed:16684826, ECO:0000269|PubMed:27305979, ECO:0000269|PubMed:7550241, ECO:0000269|PubMed:9024270, ECO:0000269|PubMed:9924196}. The disease is caused by mutations affecting the gene represented in this entry. |
| General Description of Thyroid Peroxidase . | |
| Thyroid peroxidase (TPO) is a transmembrane protein of 107kDa containing a heme prosthetic group. It is present as a dimer on the apical surface of thyroid follicular cells. TPO is the primary enzyme involved in thyroid hormone synthesis. Malignant thyroid tumors exhibit an anomaly in TPO resulting in lower affinity for anti-TPO. This antibody may aid in the differentiation between benign and malignant thyroid tumors. | |
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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