PDF DatasheetHuman, Mouse, and Rat Anti-TRAcP Antibody Product Attributes
Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-TRAcP Antibody Clone: SPM601
Clone SPM601 Host and Isotype: Mouse IgG2b kappa
Anti-Human, Mouse, and Rat TRAcP Positive Control Sample: HepG2, 293T, K562 or RPMI-8226 Cells. Spleen from Hairy Cell Leukemia (HCL) patient.
Cellular Localization of Antibody Cytoplasmic (Lysosomes)
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human ACP5 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
TRAcP Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-ACP5 antibody staining is expected to be primarily localized to the cytosol.
| TRAcP General Information | |
|---|---|
| Alternate Names | |
| Tartrate-resistant acid phosphatase, TRAP, TRAPase, acid phosphatase 5, tartrate resistant, ACP5 | |
| Molecular Weight | |
| 35kDa | |
| Chromosomal Location | |
| 19p13.2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ACP5 |
| Entrez Gene ID | 54 |
| Ensemble Gene ID | ENSG00000102575 |
| RefSeq Protein Accession(s) | NP_001104506, NP_001104505, NP_001308952, XP_011526371, NP_001104504, XP_005259995, NP_001602 |
| RefSeq mRNA Accession(s) | NM_001611, XM_005259938, NM_001322023, XM_011528069, NM_001111034, NM_001111035, NM_001111036 |
| RefSeq Genomic Accession(s) | NG_028127, NC_018930, NC_000019 |
| UniProt ID(s) | P13686, A0A024R7F8 |
| UniGene ID(s) | P13686, A0A024R7F8 |
| HGNC ID(s) | 124 |
| Cosmic ID(s) | ACP5 |
| KEGG Gene ID(s) | hsa:54 |
| PharmGKB ID(s) | PA24448 |
| General Description of TRAcP. | |
| It recognizes a protein of 35kDa, which is identified as tartrate-resistant acid phosphatase (TRAcP). It exists as two isoforms (5a, 5b). This MAb reacts with both the isoforms. Serum TRAcP 5a is secreted by macrophages, dendritic cells, increased in many patients of rheumatoid arthritis.Serum TRAcP 5b is produced from osteoclasts, elevated during bone resorption. TRAcP is an iron containing glycoprotein, which catalyzes the conversion of orthophosphoric monoester to alcohol, orthophosphate. It is the most basic of the acid phosphatases, is the only form not inhibited by L(+)-tartrate. TRAcP is synthesized as a latent proenzyme, is activated by proteolytic cleavage, reduction. Normally, TRAcP is highly expressed by osteoclasts, activated macrophages, neurons, endometrium during pregnancy. Expression of TRAcP is increased in certain pathological conditions such as Leukemic Reticuloendotheliosis (Hairy Cell Leukemia), Gaucher s Disease, HIV-induced Encephalopathy, Osteoclastoma, in osteoporosis, metabolic bone diseases. Anti-TRAcP antibody labels the cells of Hairy Cell Leukemia (HCL) with a high degree of sensitivity, specificity. Other cells stained with this antibody are tissue macrophages, osteoclasts. | |
Limitations and Warranty
enQuire Bio’s TRAcP Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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