PDF DatasheetAntibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | EDTA Buffer pH 8.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Monoclonal
Anti-Tyrosinase, Melanoma Marker Antibody Clone: T311
Host and Isotype: Mouse IgG2a
Recommended Positive Control Sample: Melanoma
Cellular Localization of Antibody T311 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with a recombinant tyrosinase protein.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
Tyrosinase, Melanoma Marker Information for Pathologists
Summary:
Also called T311 (for immunohistochemistry). Melanocyte specific antigen important in melanin synthesis and melanosome formation. Interpretation: cytoplasmic staining. Uses by pathologists Identification of melanocytic neoplasms.
Common Uses By Pathologists:
Identification of melanocytic neoplasms. Microscopic (histologic) images Images hosted on other servers:. Normal skin. prominent melanocytes (a);.
| Tyrosinase, Melanoma Marker General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 60.4 kDa | |
| Chromosomal Location | |
| q14.3 [chr: 11] [chr_start: 89177875] [chr_end: 89295759] [strand: 1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | TYR |
| Entrez Gene ID | 7299 |
| RefSeq Protein Accession(s) | NP_000363 |
| RefSeq mRNA Accession(s) | NM_000372; XM_011542970; |
| RefSeq Genomic Accession(s) | NC_000011; NG_008748 |
| UniProt ID(s) | P14679 |
| PharmGKB ID(s) | PA37095 |
| KEGG Gene ID(s) | hsa:7299 |
| Associated Diseases (KEGG IDs) | Albinism, oculocutaneous, 1A (OCA1A) [MIM:203100]: An autosomal recessive disorder in which the biosynthesis of melanin pigment is absent in skin, hair, and eyes. It is characterized by complete lack of tyrosinase activity due to production of an inactive enzyme. Patients present with a life-long absence of melanin pigment after birth, and manifest increased sensitivity to ultraviolet radiation with predisposition to skin cancer. Visual anomalies include decreased acuity, nystagmus, strabismus and photophobia. {ECO:0000269|PubMed:10571953, ECO:0000269|PubMed:10671066, ECO:0000269|PubMed:10987646, ECO:0000269|PubMed:11295837, ECO:0000269|PubMed:11858948, ECO:0000269|PubMed:1487241, ECO:0000269|PubMed:15146472, ECO:0000269|PubMed:1642278, ECO:0000269|PubMed:1899321, ECO:0000269|PubMed:1943686, ECO:0000269|PubMed:1970634, ECO:0000269|PubMed:22981120, ECO:0000269|PubMed:2342539, ECO:0000269|PubMed:23504663, ECO:0000269|PubMed:24934919, ECO:0000269|PubMed:7902671, ECO:0000269|PubMed:7955413, ECO:0000269|PubMed:8128955, ECO:0000269|PubMed:8644824, ECO:0000269|PubMed:9259202}. The disease is caused by mutations affecting the gene represented in this entry.; Albinism, oculocutaneous, 1B (OCA1B) [MIM:606952]: An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. It is characterized by partial lack of tyrosinase activity. Patients have white hair at birth that rapidly turns yellow or blond. They manifest the development of minimal-to-moderate amounts of cutaneous and ocular pigment. Some patients may have with white hair in the warmer areas (scalp and axilla) and progressively darker hair in the cooler areas (extremities). This variant phenotype is due to a loss of tyrosinase activity above 35-37 degrees C. {ECO:0000269|PubMed:10987646, ECO:0000269|PubMed:1900309, ECO:0000269|PubMed:1903591, ECO:0000269|PubMed:8128955}. The disease is caused by mutations affecting the gene represented in this entry. |
| General Description of Tyrosinase, Melanoma Marker . | |
| This antibody is specific to a cluster of proteins between 70-80 kDa known as tyrosinase. This antibody does not show any cross-reaction with MAGE-1 and tyrosinase-related protein-1, TRP-1/gp75. Staining of melanomas with this antibody showed tyrosinase in melanotic as well as amelanotic variants. | |
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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