.jpg "VEGF IHC human Ovarian Carcinoma (SPM225)")
PDF DatasheetHuman, Mouse, Rat, Rabbit, and Canine Anti-VEGF Antibody Product Attributes
VEGF Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in VEGF antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in respiratory epithelial cells in the bronchus, glandular cells in the colon, duodenum, endometrium and gallbladder, hepatocytes in liver, macrophages in lung, exocrine glandular cells in the pancreas, decidual cells in the placenta, trophoblastic cells in the placenta, glandular cells in the prostate, rectum, salivary gland, seminal vesicle and small intestine, chondrocytes in mesenchymal tissue, fibroblasts in mesenchymal tissue, glandular cells in the stomach, Leydig cells in the testis, glandular cells in the thyroid gland, squamous epithelial cells in the tonsil and urothelial cells in the urinary bladder. More moderate antibody staining intensity was present in respiratory epithelial cells in the bronchus, glandular cells in the colon, duodenum, endometrium and gallbladder, hepatocytes in liver, macrophages in lung, exocrine glandular cells in the pancreas, decidual cells in the placenta, trophoblastic cells in the placenta, glandular cells in the prostate, rectum, salivary gland, seminal vesicle and small intestine, chondrocytes in mesenchymal tissue, fibroblasts in mesenchymal tissue, glandular cells in the stomach, Leydig cells in the testis, glandular cells in the thyroid gland, squamous epithelial cells in the tonsil and urothelial cells in the urinary bladder. Low, but measureable presence of VEGF could be seen in cells in the molecular layer in cerebellum, endothelial cells in the cerebral cortex, neuropil in cerebral cortex, endothelial cells in the colon, smooth muscle cells in the smooth muscle, adipocytes in mesenchymal tissue, cells in the red pulp in spleen and cells in the white pulp in spleen. We were unable to detect VEGF in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of VEGF expression as measured by anti-VEGF antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | ++ | ++ | ++ | +++ | +++ | ++ | ++ | +++ | +++ | + | ++ | ++ | ++ | +++ | ++ | +++ | ++ | – | ++ | ++ |
| VEGFA Variability | ++ | ++ | ++ | + | ++ | +++ | ++ | + | + | ++ | ++ | +++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
| VEGF General Information | |
|---|---|
| Alternate Names | |
| Vascular endothelial growth factor A, VEGF-A, VEGFA | |
| Molecular Weight | |
| 19-22kDa (reducing), 38kDa-44kDa (non-reducing) | |
| Chromosomal Location | |
| 6p12 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | VEGFA |
| Entrez Gene ID | 7422 |
| Ensemble Gene ID | ENSG00000112715 |
| RefSeq Protein Accession(s) | NP_001165094, NP_001165101, NP_001273973, NP_003367, NP_001020541, NP_001165093, NP_001165095, NP_001165098, NP_001020538, NP_001028928, NP_001165097, NP_001165099, NP_001303939, NP_001020539, NP_001165100, NP_001191314, NP_001020537, NP_001020540, NP_001165096, NP_001191313 |
| RefSeq mRNA Accession(s) | NM_001025367, NM_001025369, NM_001171627, NM_001204384, NM_001317010, NM_003376 NM_001025368, NM_001171623, NM_001287044, NM_001033756, NM_001171628, NM_001171626, NM_001171630, NM_001171625, NM_001171629, NM_001025366, NM_001025370, NM_001171622, NM_001204385, NM_001171624 |
| RefSeq Genomic Accession(s) | NC_000006, NC_018917, NG_008732 |
| UniProt ID(s) | A0A024RD37, A0A024RD33, A0A087X1L1, A0A0Y0IMM4, A0A087WUD8, A2A2V4, A0A087X0B2, A0A087WTP4, P15692 |
| UniGene ID(s) | A0A024RD37, A0A024RD33, A0A087X1L1, A0A0Y0IMM4, A0A087WUD8, A2A2V4, A0A087X0B2, A0A087WTP4, P15692 |
| HGNC ID(s) | 12680 |
| Cosmic ID(s) | VEGFA |
| KEGG Gene ID(s) | hsa:7422 |
| PharmGKB ID(s) | PA37302 |
| General Description of VEGF. | |
| This MAb recognizes proteins of 19-22kDa (reducing), 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-,, 121-amino acid residues. The smaller two isoforms, VEGF165, VEGF121, are secreted proteins, act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression, metastasis. | |
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