Human Anti-Bcl-2 (Apoptosis& Follicular Lymphoma Marker) Antibody Product Attributes
Bcl-2 (Apoptosis& Follicular Lymphoma Marker) Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-BCL2 antibody staining is expected to be primarily localized to the nuclear membrane and nucleoplasm.
Observed Antibody Staining Data By Tissue Type:
Variations in Bcl-2 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the endometrial stroma in endometrium, cells in the white pulp in spleen, glandular cells in the endometrium, epididymis, fallopian tube, prostate, seminal vesicle and thyroid gland, hematopoietic cells in the bone marrow, lymphoid tissue in appendix, melanocytes in skin, non-germinal center cells in the lymph node and tonsil, ovarian stroma cells in the ovary, peripheral nerve/ganglion in colon, respiratory epithelial cells in the bronchus and nasopharynx and trophoblastic cells in the placenta. More moderate antibody staining intensity was present in cells in the endometrial stroma in endometrium, cells in the white pulp in spleen, glandular cells in the endometrium, epididymis, fallopian tube, prostate, seminal vesicle and thyroid gland, hematopoietic cells in the bone marrow, lymphoid tissue in appendix, melanocytes in skin, non-germinal center cells in the lymph node and tonsil, ovarian stroma cells in the ovary, peripheral nerve/ganglion in colon, respiratory epithelial cells in the bronchus and nasopharynx and trophoblastic cells in the placenta. Low, but measureable presence of Bcl-2 could be seen inadipocytes in breast and mesenchymal tissue, exocrine glandular cells in the pancreas, glandular cells in the duodenum, gallbladder and small intestine and squamous epithelial cells in the esophagus. We were unable to detect Bcl-2 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of Bcl-2 expression as measured by anti-Bcl-2 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Signal Intensity | ++ | – | – | – | + | – | – | – | – | +++ | +++ | – | – | – | ++ | – | – | – | – | – |
BCL2 Variability | ++ | ++ | + | ++ | ++ | ++ | + | + | ++ | ++ | ++ | ++ | ++ | + | ++ | ++ | + | + | ++ | + |
Bcl-2 (Apoptosis& Follicular Lymphoma Marker) General Information | |
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Alternate Names | |
Apoptosis regulator Bcl-2, B-cell CLL/lymphoma-2 | |
Molecular Weight | |
25-26kDa | |
Chromosomal Location | |
Ships on blue ice. | |
Curated Database and Bioinformatic Data | |
Gene Symbol | 596 |
Entrez Gene ID | BCL2 |
UniProt ID(s) | P10415 |
UniGene ID(s) | Hs150749 |
COSMIC ID Link(s) | BCL2 |
KEGG Gene ID(s) | hsa:596 |
General Description of Bcl-2 (Apoptosis& Follicular Lymphoma Marker). | |
This antibody recognizes a protein of 25-26kDa, identified as the bcl-2lpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression ofbcl-2lpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2lpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative. |
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