Bcl-2 (Apoptosis& Follicular Lymphoma Marker) Ultraspecific Antibody

Tested against >20,000 Human Proteins

$ 199.00$ 429.00

SKU: 596-MSM11
Species: Human
Tested Applications: Flow, IF, WB, IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG1, kappa
Mass Spec Validated?: Not MS Validated

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Human Anti-Bcl-2 (Apoptosis& Follicular Lymphoma Marker) Antibody Product Attributes

Species: Human
Tested Applications: Flow, IF, WB, IHC.
Clonality: Monoclonal
Anti-Bcl-2 (Apoptosis& Follicular Lymphoma Marker) Antibody Clone: rBCL2/782
Clone rBCL2/782 Host and Isotype: Mouse IgG1, kappa
Anti-Human Bcl-2 (Apoptosis& Follicular Lymphoma Marker) Positive Control Sample: Jurkat, K562, HL-60, or HeLa Cells. Tonsil or follicular lymphomas.
Cellular Localization of Antibody rBCL2/782 Staining: Outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes.
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.
Immunogen: Recombinant full-length human bcl-2 protein
Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

Bcl-2 (Apoptosis& Follicular Lymphoma Marker) Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-BCL2 antibody staining is expected to be primarily localized to the nuclear membrane and nucleoplasm.

Observed Antibody Staining Data By Tissue Type:

Variations in Bcl-2 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the endometrial stroma in endometrium, cells in the white pulp in spleen, glandular cells in the endometrium, epididymis, fallopian tube, prostate, seminal vesicle and thyroid gland, hematopoietic cells in the bone marrow, lymphoid tissue in appendix, melanocytes in skin, non-germinal center cells in the lymph node and tonsil, ovarian stroma cells in the ovary, peripheral nerve/ganglion in colon, respiratory epithelial cells in the bronchus and nasopharynx and trophoblastic cells in the placenta. More moderate antibody staining intensity was present in cells in the endometrial stroma in endometrium, cells in the white pulp in spleen, glandular cells in the endometrium, epididymis, fallopian tube, prostate, seminal vesicle and thyroid gland, hematopoietic cells in the bone marrow, lymphoid tissue in appendix, melanocytes in skin, non-germinal center cells in the lymph node and tonsil, ovarian stroma cells in the ovary, peripheral nerve/ganglion in colon, respiratory epithelial cells in the bronchus and nasopharynx and trophoblastic cells in the placenta. Low, but measureable presence of Bcl-2 could be seen inadipocytes in breast and mesenchymal tissue, exocrine glandular cells in the pancreas, glandular cells in the duodenum, gallbladder and small intestine and squamous epithelial cells in the esophagus. We were unable to detect Bcl-2 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Bcl-2 expression as measured by anti-Bcl-2 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ + +++ +++ ++
BCL2 Variability ++ ++ + ++ ++ ++ + + ++ ++ ++ ++ ++ + ++ ++ + + ++ +

Limitations and Warranty

enQuire Bio’s product, Bcl-2 (Apoptosis& Follicular Lymphoma Marker) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.
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Bcl-2 (Apoptosis& Follicular Lymphoma Marker) Ultraspecific Antibody

Tested against >20,000 Human Proteins

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