PDF DatasheetHuman Anti-PU.1 (SPI-1) (B-Cell Marker) Antibody Product Attributes
Species: Human
Tested Applications: WB, IHC.
Clonality: Monoclonal
Anti-PU.1 (SPI-1) (B-Cell Marker) Antibody Clone: PU1/2146
Clone PU1/2146 Host and Isotype: Mouse IgG2b, kappa
Anti-Human PU.1 (SPI-1) (B-Cell Marker) Positive Control Sample: K-562 Cells, THP-1 cells. Lymph Node, Spleen, Hodgkin s Lymphoma, Colon Carcinoma.
Cellular Localization of Antibody PU1/2146 Staining: Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.
Immunogen: Recombinant fragment (apprx. aa 16-170) of human PU.1 protein (Please call for additional information.)
Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.
PU.1 (SPI-1) (B-Cell Marker) Previously Observed Antibody Staining Patterns
| PU.1 (SPI-1) (B-Cell Marker) General Information | |
|---|---|
| Alternate Names | |
| Transcription Factor spi1; 31kDa Transforming Protein; Hematopoietic Transcription Factor PU.1; SFPI1; SPI1; SPIA; Spleen focus forming virus (SFFV) proviral integration oncogene spi1 | |
| Molecular Weight | |
| 40kDa | |
| Chromosomal Location | |
| Ships on blue ice. | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | 6688 |
| Entrez Gene ID | SPI1 |
| UniProt ID(s) | P17947 |
| UniGene ID(s) | Hs502511 |
| COSMIC ID Link(s) | SPI1 |
| KEGG Gene ID(s) | hsa:6688 |
| General Description of PU.1 (SPI-1) (B-Cell Marker). | |
| PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development. | |
Limitations and Warranty
enQuire Bio’s product, PU.1 (SPI-1) (B-Cell Marker) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.
There are no reviews yet.