PDF DatasheetHuman Bcl 2 (1-206 a. a. ) Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Bcl 2 (1-206 a. a. ) based upon sequence from Human
Host: QP10490 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of Bcl 2 (1-206 a. a. ) was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: Suspend Bcl-2 in 100ul of 0.5M Acetic acid, over night at 4°C. Dilute 10 fold into selected buffer system. BCL-2 has tendency to form intramolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended.
Bioactivity Data: Untested
Amino Acid Sequence: MANUAL
Purity: Greater than 95.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The protein contains 10mM Tris-HCl pH 8.0, 1mM EDTA and 250mM NaCl.
Storage Conditions: Lyophilized Bcl-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Bcl-2 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human Bcl 2 (1-206 a. a. ) General Information | |
|---|---|
| Alternate Names | |
| Apoptosis regulator Bcl-2, BCL2, B-cell CLL/lymphoma 2, Bcl-2. | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P10415 |
| General Description of Recombinant Human Bcl 2 (1-206 a. a. ). | |
| Human Bcl-2 produced in E. Coli is a single, non-glycosylated polypeptide chain containing amino acids 1-206. The wild type Bcl-2 is missing 12 amino acids from C-terminus. C-terminus is fused to His-Tag. C-terminus his-tag, mimics the deleted C-terminus membrane domain thus maintaining its biological activity. Bcl-2 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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