PDF DatasheetHuman APOE3 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant APOE3 based upon sequence from Human
Host: QP10522 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of APOE3 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized APOE in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Immobilized rHuApoE3 binds to rMuVLDLR with EC50 less than 0.4µg/ml.
Amino Acid Sequence: MHHHHHHKVE QAVETEPEPE LRQQTEWQSG QRWELALGRF WDYLRWVQTL SEQVQEELLS SQVTQELRAL MDETMKELKA YKSELEEQLT PVAEETRARL SKELQAAQAR LGADMEDVCG RLVQYRGEVQ AMLGQSTEEL RVRLASHLRK LRKRLLRDAD DLQKRLAVYQ AGAREGAERG LSAIRERLGP LVEQGRVRAA TVGSLAGQPL QERAQAWGER LRARMEEMGS RTRDRLDEVK EQVAEVRAKL EEQAQQIRLQ AEAFQARLKS WFEPLVEDMQ RQWAGLVEKV QAAVGTSAAP VPSDNH
Purity: Greater than 95% as determined by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: Lyophilized from a 0.2µm filtered solution in PBS.
Storage Conditions: Lyophilized APOE3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution APOE should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human APOE3 General Information | |
|---|---|
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| Alternate Names | |
| Apolipoprotein E, LDLCQ5, APO-E, LPG, AD2, Alzheimer Disease 2 (APOE*E4-Associated, Late Onset), Apolipoprotein E3, APOE. | |
| Molecular Weight | |
| 35 kDa | |
| Chromosomal Location | |
| q13.32 on chromosome 19 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | APOE |
| Entrez Gene ID | 348 |
| UniProt ID(s) | P02649 |
| COSMIC ID Link(s) | APOE |
| KEGG Gene ID(s) | hsa:348 |
| General Description of Recombinant Human APOE3. | |
| Human APOE3 (19-317) produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 306 amino acids and having a molecular mass of 35kDa. The APOE is fused to a 7 amino acid His tag [M-HHHHHH] at N-terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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