PDF Datasheetother Avidin Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Avidin based upon sequence from other
Host: QP10525 protein expressed in Native Protein.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Avidin was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes Avidin may be used to visualize biotin conjugated molecules in ELISA, blotting and histological techniques.
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Avidin in sterile 18MΩ.cm H2O not less than 100µg/ml or more than 10 mg/ml solutions.
Bioactivity Data: 16 units/mg protein, 1 unit binds 1µg biotin.
Amino Acid Sequence: MVHATSPLLL LLLLSLALVA PGLSARKCSL TGKWTNDLGS NMTIGAVNSR GEFTGTYITA VTATSNEIKE SPLHGTQNTI NKRTQPTFGF TVNWKFSEST TVFTGQCFID RNGKEVLKTM WLLRSSVNDI GDDWKATRVG INIFTRLRTQ KE
Purity: Greater than 92.0%.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Storage Conditions: Lyophilized Avidin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Avidin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered white lyophilized powder.
| Recombinant other Avidin General Information | |
|---|---|
| Alternate Names | |
| Avidin, AVD, AVID. | |
| Molecular Weight | |
| 68 kDa | |
| Chromosomal Location | |
| on chromosome Z | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | AVD |
| Entrez Gene ID | 396260 |
| UniProt ID(s) | P02701 |
| COSMIC ID Link(s) | AVD |
| KEGG Gene ID(s) | gga:396260 |
| General Description of Recombinant other Avidin. | |
| Avidin is a glycosylated polypeptide chain having a molecular mass of 68kDa and containing 4 subunits each with a binding site for biotin. The Avidin is purified by affinity chromatographic techniques. The purification procedure ensures minimal contamination by other proteins or DNA. The resulting high activity and purity of the product gives very low non-specific binding (NSB). | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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