PDF Datasheetother Avidin Biotin Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Avidin Biotin based upon sequence from other
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Avidin Biotin was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: We highly recommended to prepare a 1 mg/ml solution; Reconstitute using cold deionized pyrogen free water and mix gently until powder is completely dissolved.
Why We Recommend this protein: 1. Avidin-Biotin does not exhibit unwanted non-specific binding that have been previously reported for the native egg white avidin. 2. The non-specific properties of Avidin-Biotin have been tested in ELISA, Western Blot & Immunohistochemistry techniques and have been found to be superior to unmodified avidin. 3. Avidin-Biotin rarely affected by pH values between the range of pH 2 to pH 12 or by guanidine-HCl concentrations up to 8M at neutral pH.
Bioactivity Data: Binding activity of Avidin-Biotin is 10-15units/mg protein. One unit of Avidin activity is defined as the amount of protein which will bind 1µg d-biotin.
Amino Acid Sequence: MVHATSPLLL LLLLSLALVA PGLSARKCSL TGKWTNDLGS NMTIGAVNSR GEFTGTYITA VTATSNEIKE SPLHGTQNTI NKRTQPTFGF TVNWKFSEST TVFTGQCFID RNGKEVLKTM WLLRSSVNDI GDDWKATRVG INIFTRLRTQ KE
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: Salt-free lyophilized powder with no preservative added.
Storage Conditions: Store protein solution at 2-8°C.
Physical Appearance: Lyophilized freeze dry Powder.
| Recombinant other Avidin Biotin General Information | |
|---|---|
| Alternate Names | |
| Avidin, AVD, AVID. | |
| Chromosomal Location | |
| on chromosome Z | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | AVD |
| Entrez Gene ID | 396260 |
| UniProt ID(s) | P02701 |
| COSMIC ID Link(s) | AVD |
| KEGG Gene ID(s) | gga:396260 |
| General Description of Recombinant other Avidin Biotin. | |
| Avidin is a basic charged glycoprotein present in the egg white and in some extent in tissues of various animals. Avidin is a homotetrameric protein that contains 4 identical subunits which bind to Biotin with high extent of affinity and specificity consisting of 128 amino acids and has a molecular mass of about 67K Daltons. Avidin-Biotin is a modified form of affinity purified avidin that combines the high affinity specific activity of the native egg white avidin to biotin molecule together with a very low background of the streptavidin produced by the bacteria Streptomyces avidinii. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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