PDF DatasheetMouse b NGF Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant b NGF based upon sequence from Mouse
Host: QP10528 protein expressed in Native Protein, E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of b NGF was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Murine NGF-beta in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The method used to test the bioassay is the NGF-dependent survival of dorsal root ganglia neurons of chick embryo, corresponding to a Specific Activity of 500,000IU/mg.
Monomer or Dimer: Dimer
Amino Acid Sequence: SSTHPVFHMGEF SVCDSVSVWV GDKTTATDIK GKEVTVLAEV NINNSVFRQY FFETKCRASN PVESGCRGID SKHWNSYCTT THTFVKALTT DEKQAAWRFI RIDTACVCVL SRKATRRG
Purity: Greater than 98% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE .
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The NGF beta Mouse was lyophilized from solution containing 5% mannitol and 1% HSA.
Storage Conditions: Lyophilized Mouse Beta-NGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Murine NGF-Beta should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Mouse b NGF General Information | |
|---|---|
| Alternate Names | |
| Beta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428. | |
| Molecular Weight | |
| 13.5 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P01139 |
| General Description of Recombinant Mouse b NGF. | |
| NGF beta Mouse produced in Submaxillary Gland of Grown Mouse is a homodimer, non-glycosylated, polypeptide chain containing 2 identical 120 amino acids and having a molecular mass of 13,471 Dalton each. The NGF beta Mouse is purified by advanced biology purification technology. | |
Selected References
V aron S, Raibo rn C. Dissociation, fractionation and culture ofchick embryo sympathetic ganglionic cells [ J ]. J N eu rocy tol, 1972; 1: 211- 221.
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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