PDF Datasheetother Batroxobin Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Batroxobin based upon sequence from other
Host: QP10529 protein expressed in Pichia Pastoris.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Batroxobin was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Batroxobin in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Batroxobins biological activity was found to be of no less than 500KU/mg. (Klobusitzky Unit). One KU is defined as the amount of enzyme which coagulates standard human plasma incubated at 37°C in vitro within 60 ± 20 seconds.
Amino Acid Sequence: VIGGDECDIN EHPFLAFMYY SPRYFCGMTL INQEWVLTAA HCNRRFMRIH LGKHAGSVAN YDEVVRYPKE KFICPNKKKN VITDKDIMLI RLDRPVKNSE HIAPLSLPSN PPSVGSVCRI MGWGAITTSE DTYPDVPHCA NINLFNNTVC REAYNGLPAK TLCAGVLQGG IDTCGGDSGG PLICNGQFQG ILSWGSDPCA EPRKPAFYTK VFDYLPWIQS IIAGNKTATC P
Purity: Greater than 97.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The Batroxobin protein was lyophilized from a concentrated (1 mg/ml) solution containing 20mM sodium acetate buffer, pH 7.4.
Storage Conditions: Batroxobin although stable at room temperature for 3 weeks, should be stored below -18°C. Upon reconstitution Batroxobin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered white lyophilized powder.
| Recombinant other Batroxobin General Information | |
|---|---|
| Alternate Names | |
| Thrombin-like enzyme batroxobin, EC 3.4. 21.74, BX, Bothrops atrox serine proteinase, Venombin-A, Defibrase, Reptilase, Batroxobin. | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P04971 |
| General Description of Recombinant other Batroxobin. | |
| The Batroxobin Recombinant Protein, produced in yeast, is a single, glycosylated polypeptide chain containing 231 amino acids and having an MW of approximately 28-33 kDa. | |
Selected References
Impaired clot retraction in factor XIII A subunit-deficient mice Supplemental materials for: Kasahara et alPublication:Published online before print December 8, 2009, doi: 10.1182/blood-2009-06-227645 Blood December 8, 2009 Link:http://bloodjournal.hematologylibrary.org/content/115/6/1277/suppl/DC1
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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