PDF DatasheetHuman BMP10 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant BMP10 based upon sequence from Human
Host: QP10536 protein expressed in HEK 293.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of BMP10 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized BMP10 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The specific activity was determined by its ability to induce alkaline phosphatase production in the ATDC-5 cell line and is typically 4.0-6.0 ng/ml ED50.
Amino Acid Sequence: NAKGNYCKRT PLYIDFKEIG WDSWIIAPPG YEAYECRGVC NYPLAEHLTP TKHAIIQALVHLKNSQKASK ACCVPTKLEP ISILYLDKGV VTYKFKYEGM AVSECGCR
Purity: Greater than 95.0% as determined by analysis by SDS-PAGE and HPLC analysis.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The BMP10 protein was lyophilized from 10mM Sodium Citrate, pH 2.7 and 75mM NaCl .
Storage Conditions: Lyophilized BMP10 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BMP10 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human BMP10 General Information | |
|---|---|
| Alternate Names | |
| Bone morphogenetic protein 10, BMP-10. | |
| Molecular Weight | |
| 26.2 kDa | |
| Chromosomal Location | |
| p13.3 on chromosome 2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | BMP10 |
| Entrez Gene ID | 27302 |
| UniProt ID(s) | O95393 |
| COSMIC ID Link(s) | BMP10 |
| KEGG Gene ID(s) | hsa:27302 |
| General Description of Recombinant Human BMP10. | |
| Human BMP10 produced in HEK cells having a molecular weight of 26.2kDa. BMP10 Human is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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