PDF DatasheetBovine BTC Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant BTC based upon sequence from Bovine
Host: QP10540 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of BTC was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized BTC Bovine in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The ED50, calculated by the dose-dependent proliferation of murine BALBC 3T3 cells (measured by 3H-thymidine uptake) is < 10.0 ng/ml, corresponding to a Specific Activity 100,000 units/mg.
Amino Acid Sequence: MARAAPGSGA SPLPLLPALA LGLVILHCVV ADGNSTRSPE DDGLLCGDHA ENCPATTTQP KRRGHFSRCP KQYKHYCIKG RCRFVVAEQT PSCVCDEGYA GARCERVDLF YLRGDRGQIL VICLIAVMVI FIILVVSICT CCHPLRKRRK RRKKEEEMET LGKDITPIND DIQETSIA
Purity: Greater than 95.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Concentration of Bovine BTC Protein: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.59 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of BTC as a Reference Standard.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The Betacellulin Bovine Recombinant was lyophilized after extensive dialysis against 50mM acetic acid.
Storage Conditions: Lyophilized Betacellulin Bovine Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BTC Bovine should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Bovine BTC General Information | |
|---|---|
 |
|
| Molecular Weight | |
| 9 kDa | |
| Chromosomal Location | |
| On chromosome 6 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | BTC |
| Entrez Gene ID | 280737 |
| UniProt ID(s) | Q9TTC5 |
| COSMIC ID Link(s) | BTC |
| KEGG Gene ID(s) | bta:280737 |
| General Description of Recombinant Bovine BTC. | |
| Betacellulin Bovine Recombinant produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 80 amino acids and having a molecular mass of 9003 Dalton. Betacellulin Bovine Recombinant is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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