PDF DatasheetHuman C-JUN Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant C-JUN based upon sequence from Human
Host: QP10551 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of C-JUN was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to centrifuge the vial prior to opening in order to bring the contents to the bottom. The reconstitution of the lyophilized c-Jun is recommended in 40mM Tris, pH 7.5, to a concentration of 0.2-1.0 mg/ml.
Additional Information: Kinase activity may vary depending on the substrate and reaction conditions.
Bioactivity Data: C-Jun is phosphorylatable in vitro, using either recombinant active JNK1 or JNK2, or with JNK immunoprecipitated from stimulated cells. This phosphorylation can be monitored by Western blot analysis using an antibody directed to c-Jun [pS73], in conjunction with chemiluminescence detection methods. Optimization of the cell stimulation protocol, cell lysis procedure, and reaction conditions may be required for each specific application.
Amino Acid Sequence: MTAKMETTFY DDALNASFLP SESGPYGYSN PKILKQSMTL NLADPVGSLK PHLRAKNSDL LTSPDVGLLK LASPELERLI IQSSNGHITT TPTPTQFLCP KNVTDEQEGF AEGFVRALAE LHSQNTLPSV TSAAQPVNGA GMVAPAVASV AGGSGSGGFS ASLHSEPPVY ANLSNFNPGA LSSGGGAPSY GAAGLAFPAQ PQQQQQPPHH LPQQMPVQHP RLQALKEEPQ TVPEMPGETP PLSPIDMESQ ERIKAERKRM RNRIAASKCR KRKLERIARL EEKVKTLKAQ NSELASTANM LREQVAQLKQ KVMNHVNSGC QLMLTQQLQT F
Purity: Greater than 95% as determined by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: C-JUN is supplied as lyophilized powder containing no additives.
Storage Conditions: Store at 4°C if entire vial will be used within 1-2 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human C-JUN General Information | |
|---|---|
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| Alternate Names | |
| Transcription factor AP-1, Activator protein 1, AP1, Proto-oncogene c-jun, V-jun avian sarcoma virus 17 oncogene homolog, p39, c-Jun. | |
| Molecular Weight | |
| 52 kDa | |
| Chromosomal Location | |
| p32.1 on chromosome 1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | JUN |
| Entrez Gene ID | 3725 |
| UniProt ID(s) | P05412 |
| COSMIC ID Link(s) | JUN |
| KEGG Gene ID(s) | hsa:3725 |
| General Description of Recombinant Human C-JUN. | |
| C-JUN amino acids 1-81 produced in E. coli, is a non-glycosylated, polypeptide chain having a molecular mass of 52 kDa. C-JUN is a maltose binding protein (MBP) fusion protein with an amino-terminal polyhistidine tag and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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