PDF DatasheetHuman CST3 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant CST3 based upon sequence from Human
Host: QP10561 protein expressed in HEK 293.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of CST3 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Cystatin-C in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The inhibitory function of Cystatin-C on papins protease activity was measured by a colorimetric assay using L-BAPA as substrate. IC50 value was measured at 5-20 µg/ml (0.3-1.5 µM) with a range of 1.56-50µg/ml Cystatin-C in presence of 0.55µM papain and 0.44µM L-BAPA. The activity is typically 0.3-1.5µM IC50.
Purity: Greater than 95% as obsereved by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The Cystatin-C was lyophilized from 1 mg/ml in 1xPBS.
Storage Conditions: Lyophilized Cystatin-C although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Cystatin-C should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human CST3 General Information | |
|---|---|
| Alternate Names | |
| Cystatin-C, Cystatin-3, Neuroendocrine basic polypeptide, Gamma-trace, Post-gamma-globulin, CST3, MGC117328. | |
| Molecular Weight | |
| approximately 13 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q6FGW9 |
| General Description of Recombinant Human CST3. | |
| Human Cystatin-C produced in HEK cells is a non-glycosylated monomer, having a molecular weight of approximately 13kDa. The Cystatin-C is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bios products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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