PDF DatasheetHuman DPP4 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant DPP4 based upon sequence from Human
Host: QP10576 protein expressed in Insect, Native Protein.
Available Tags: His, Untagged
Protein Construction: A cDNA sequence encoding the sequence of DPP4 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Definition of Unit (for specific activity): One unit will hydrolyze 1 umole of p-nitroaniline per minute at pH8.0 at 37°C using 1mM of Gly-Pro p-nitroanilde as a substrate.
Other Applications: – Reaction buffer: 20mM Tris pH8.0, 0.1M NaCl, 1mM EDTA- Total reaction volume: 100 ul- Reaction temperature: 37°C. 1. Add the reaction buffer to each well. 2. Add the 10 ul of 10mM substrate (Gly-Pro p-nitroanilide) to each well. 3. Add the enzyme (DPP-4) diluent to each well. 4. Incubate the 96 well plate at 37°C. 5. Read the optical density at 405 nm. Optical density was measured at 405 nm after incubating enzyme solution with 1mM of p-nitroanilid as a substrate. 0ng DPP4= 0 OD at 405nm. 20ng DPP4= 0.075 OD at 405nm. 40ng DPP4= 0.125 OD at 405nm. 60ng DPP4= 0.2 OD at 405nm. 80ng DPP4= 0.275 OD at 405nm. 100ng DPP4= 0.35 OD at 405nm.
Bioactivity Data: >200 Units/mg.
Amino Acid Sequence: ADPSRKTYTL TDYLKNTYRL KLYSLRWISD HEYLYKQENN ILVFNAEYGN SSVFLENSTF DEFGHSINDY SISPDGQFIL LEYNYVKQWR HSYTASYDIY DLNKRQLITE ERIPNNTQWV TWSPVGHKLA YVWNNDIYVK IEPNLPSYRI TWTGKEDIIY NGITDWVYEE EVFSAYSALW WSPNGTFLAY AQFNDTEVPL IEYSFYSDES LQYPKTVRVP YPKAGAVNPT VKFFVVNTDS LSSVTNATSI QITAPASMLI GDHYLCDVTW ATQERISLQW LRRIQNYSVM DICDYDESSG RWNCLVARQH IEMSTTGWVG RFRPSEPHFT LDGNSFYKII SNEEGYRHIC YFQIDKKDCT FITKGTWEVI GIEALTSDYL YYISNEYKGM PGGRNLYKIQ LSDYTKVTCL SCELNPERCQ YYSVSFSKEA KYYQLRCSGP GLPLYTLHSS VNDKGLRVLE DNSALDKMLQ NVQMPSKKLD FIILNETKFW YQMILPPHFD KSKKYPLLLD VYAGPCSQKA DTVFRLNWAT YLASTENIIV ASFDGRGSGY QGDKIMHAIN RRLGTFEVED QIEAARQFSK MGFVDNKRIA IWGWSYGGYV TSMVLGSGSG VFKCGIAVAP VSRWEYYDSV YTERYMGLPT PEDNLDHYRN STVMSRAENF KQVEYLLIHG TADDNVHFQQ SAQISKALVD VGVDFQAMWY TDEDHGIASS TAHQHIYTHM SHFIKQCFSL PHHHHHH
Purity: Greater than 95.0% as determined by Analysis by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: DPP4 is formulated in 20mM Tris-HCl buffer pH 8.0, 100mM NaCl, 1mM EDTA and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human DPP4 General Information | |
|---|---|
| Alternate Names | |
| CD26, ADABP, ADCP2, DPPIV, TP103, DPP4, Dipeptidyl peptidase 4, Dipeptidyl peptidase IV, DPP IV, T-cell activation antigen CD26, Adenosine deaminase complexing protein 2, CD26 antigen. | |
| Molecular Weight | |
| 85.4 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P27487 |
| General Description of Recombinant Human DPP4. | |
| Human DPPIV produced in Insect cells is a single, glycosylated polypeptide chain containing 737 amino acids (39-766) and having a molecular mass of 85.4kDa. DPPIV is fused to a 6 His Tag at C-terminus and purified using conventional chromatography techniques. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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