PDF DatasheetRat FGF 1 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant FGF 1 based upon sequence from Rat
Host: QP10596 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of FGF 1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-acidic in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The ED50 as determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells, is less than 0.2ng/ml corresponding to a Specific Activity of 5x106IU/mg.
Amino Acid Sequence: MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
Purity: Greater than 98.0% as determined by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: Lyophilized at a concentration of 1 mg/ml in 5mM Na2PO4, pH 7.5 and 50mM NaCl.
Storage Conditions: Lyophilized Fibroblast Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-a should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Rat FGF 1 General Information | |
|---|---|
| Alternate Names | |
| Fibroblast growth factor 1, FGF-1, Acidic fibroblast growth factor, aFGF, Heparin-binding growth factor 1, HBGF-1, Fgf1, Fgfa, HBGF1. | |
| Molecular Weight | |
| 15.9 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P61149 |
| General Description of Recombinant Rat FGF 1. | |
| Rat Fibroblast Growth Factor-acidic (FGF-1) produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 141 amino acids and having a molecular mass of 15.9 kDa. The FGF acidic is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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