PDF Datasheetother HPSE Active Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant HPSE Active based upon sequence from other
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of HPSE Active was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Additional Testing: Heparanase Active Enzyme is identified by Western blot analysis with polyclonalrabbit anti-HPA1 antibodies as 2 subunits of 8-kDa and 50-kDa.
Bioactivity Data: The specific activity of Heparanase Active Enzyme in-house standard is about 0.7 Units (1 unit = 1 μmole of reducing ends of heparan sulfate substrate formed per minute per mg Heparanase Active Enzyme at 37°C). The enzymatic activity of each Heparanase Active Enzyme batch is comparable to the standard as determined by activity assay in which immobilized heparan, released due to heparanase activity, is quantified colorimetrically. Recommended reaction buffer: 20 mM Citrate Phosphate buffer, pH 5.4; 50mM NaCl; 1mM CaCl2.
Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: Heparanase Active Enzyme is supplied in20mM Acetate buffer and 750mM NaCl pH 5.4.
Storage Conditions: Store at –80ºC, avoid repeated freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
Selected References
1. Vlodavsky, I. et al.., Nat. Med. 5 (1999) 793–802.2. Vlodavsky, I and Friedman, Y., J. Clin. Invest. 108 (2001) 341–347.3. Parish, C.R. et al., Biochem. Biophys. Acta 1471 (2001) M99–M108.4. Vlodasvsky, I. et al., Cancer Metastasis Rev. 9, (1990) 203-226.5. Bashkin, P. et al.,. Biochemistry 28 (1989), 1737-1743.6. Fairbanks, M.B. et al., J. Biol. Chem. 274 (1999) 29587– 29590.7. Koliopanos, A.et al., Cancer Res. 61 (1992) 4655–4659.8. Gohji, K. et al., Int. J. Cancer 95 (2001) 295–301.
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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