PDF DatasheetHuman IFN a 2b Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant IFN a 2b based upon sequence from Human
Host: QP10674 protein expressed in HEK 293, E. Coli, S. cerevisiae.
Available Tags: Untagged, 20kD PEG
Protein Construction: A cDNA sequence encoding the sequence of IFN a 2b was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized IFN-a 2b in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The specific activity was determined by the dose-dependent cytotoxicity of the human TF-1 cell line (human erythroleukemic indicator cell line) and is typically 0.02-0.08ng/ml.
Amino Acid Sequence: MCDLPQTHSL GSRRTLMLLA QMRRISLFSC LKDRHDFGFP QEEFGNQFQK AETIPVLHEM IQQIFNLFST KDSSAAWDET LLDKFYTELY QQLNDLEACV IQGVGVTETP LMKEDSILAV RKYFQRITLY LKEKKYSPCA WEVVRAEIMR SFSLSTNLQE SLRSKE
Purity: Greater than 95% as obsereved by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The IFN-a 2b was lyophilized from 1 mg/ml in 1xPBS.
Storage Conditions: Lyophilized IFN-a 2b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-a 2b should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human IFN a 2b General Information | |
|---|---|
| Alternate Names | |
| Interferon alpha 2b, IFNA, INFA2, MGC125764, MGC125765. | |
| Molecular Weight | |
| 16 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q86UP4 |
| COSMIC ID Link(s) | – |
| General Description of Recombinant Human IFN a 2b. | |
| Human IFN-a 2b produced in HEK cells is a glycosylated monomer, having a total molecular weight of 16kDa. The IFN-a 2b is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bios products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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