PDF DatasheetOvine IFN tau Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant IFN tau based upon sequence from Ovine
Host: QP10676 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of IFN tau was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Interferon Tau in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 10,000,000IU/mg.
Amino Acid Sequence: MAFVLSLLMA LVLVSYGPGG SLGCYLSRKL MLDARENLKL LDRMNRLSPH SCLQDRKDFG LPQEMVEGDQ LQKDQAFPVL YEMLQQSFNL FYTEHSSAAW DTTLLEQLCT GLQQQLDHLD TCRGQVMGEE DSELGNMDPI VTVKKYFQGI YDYLQEKGYS DCAWEIVRVE MMRALTVSTT LQKRLTKMGG DLNSP
Purity: Greater than 95.0% as determined by both:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: Lyophilized from (1 mg/ml) solution containing PBS pH 7.4.
Storage Conditions: Lyophilized Interferon-Tau although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-Tau should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Ovine IFN tau General Information | |
|---|---|
| Alternate Names | |
| IFN-tau1, Trophoblast protein 1, TP-1, Trophoblastin, Antiluteolysin, Trophoblast antiluteolytic protein, IFN-tau, Interferon tau-1. | |
| Molecular Weight | |
| 19.9 kDa | |
| Chromosomal Location | |
| On chromosome 2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | IFNT1 |
| Entrez Gene ID | 100144750 |
| UniProt ID(s) | P56828 |
| COSMIC ID Link(s) | IFNT1 |
| KEGG Gene ID(s) | oas:100144750 |
| General Description of Recombinant Ovine IFN tau. | |
| Interferon-Tau Ovine Recombinant produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 172 amino acids and having a molecular mass of 19914.7 Dalton. The IFN-Tau is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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