PDF DatasheetRat IL 13 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant IL 13 based upon sequence from Rat
Host: QP10692 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of IL 13 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Interleukin 13 in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: ED50 range = 40ng/ml, corresponding to a specific activity of > 25,000IU/mg as determined by the dose dependent proliferation of human TF-1 cells. Optimal concentration for individual application should be determined by a dose response assay.
Amino Acid Sequence: VRRSTSPPVA LRELIEELSN ITQDQKTSLC NSSIVWSVDI TAGGFCAALE SLTNISSCNA IHRTQRILNG LCNQKASDVA SSPPDTKIEV AQFISKLLNY SKQLFRYGH
Purity: Greater than 95% as determined by SDS-PAGE.
Concentration of Rat IL 13 Protein: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.69 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IL-13 as a Reference Standard.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The protein (1 mg/ml) was lyophilized in PBS, pH 7.4.
Storage Conditions: Lyophilized Interleukin-13 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL13 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Rat IL 13 General Information | |
|---|---|
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| Alternate Names | |
| NC300, ALRH, BHR1, P600, IL-13. | |
| Molecular Weight | |
| 11.9 kDa | |
| Chromosomal Location | |
| q22 on chromosome 10 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Il13 |
| Entrez Gene ID | 116553 |
| UniProt ID(s) | P42203 |
| COSMIC ID Link(s) | Il13 |
| KEGG Gene ID(s) | rno:116553 |
| General Description of Recombinant Rat IL 13. | |
| Rat Interleukin-13 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 109 amino acids and having a molecular mass of 11.9 kDa. The IL-13 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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