PDF DatasheetMouse IL 20 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant IL 20 based upon sequence from Mouse
Host: QP10701 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of IL 20 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Interleukin-20 in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The biological activity was determined by measuring the dose dependent activation of STAT3 in human epidermal keratinocytes (HEK2a cells). Significant STAT3 activation is observed with >100ng/mL of Interleukin-20 recombinant mouse, corresponding to a specific activity of 10,000 units/mg of Interleukin-20 recombinant mouse.
Amino Acid Sequence: MKGFGLAFGL FSAVGFLLWT PLTGLKTLHL GSCVITANLQ AIQKEFSEIR DSVQAEDTNI DIRILRTTES LKDIKSLDRC CFLRHLVRFY LDRVFKVYQT PDHHTLRKIS SLANSFLIIK KDLSVCHSHM ACHCGEEAME KYNQILSHFI ELELQAAVVK ALGELGILLR WMEEML
Purity: Greater than 95.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Concentration of Mouse IL 20 Protein: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.552 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-20 as a Reference Standard.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: Lyophilized from a concentrated (1 mg/ml) solution containing no additives.
Storage Conditions: Lyophilized Interleukin-20 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL20 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Mouse IL 20 General Information | |
|---|---|
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| Alternate Names | |
| IL10D, ZCYTO10, IL-20, MGC96907, Four alpha helix cytokine Zcyto10. | |
| Molecular Weight | |
| 17.6 kDa | |
| Chromosomal Location | |
| E4 on chromosome 1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Il20 |
| Entrez Gene ID | 58181 |
| UniProt ID(s) | Q9JKV9 |
| COSMIC ID Link(s) | Il20 |
| KEGG Gene ID(s) | mmu:58181 |
| General Description of Recombinant Mouse IL 20. | |
| Mouse Interleukin-20 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids and having a total molecular mass of 17,584 Dalton. The IL-20 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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