PDF DatasheetRat IL 21 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant IL 21 based upon sequence from Rat
Host: QP10702 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of IL 21 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Rat IL21 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The biological activity is measured by its ability to proliferate activated B cells.
Amino Acid Sequence: HKSSPQRPDH LLIRLRHLMD IVEQLKIYEN DLDPELLTAP QDVKGQCEHE AFACFQKAKL KPSNTGNNKT FINDLLAQLR RRLPAKRTGN KQRHMAKCPS CDLYEKKTPK EFLERLKWLL QKMIHQHLS
Purity: Greater than 95.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: Rat IL-21 was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.
Storage Conditions: Lyophilized Rat IL-21 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Rat IL21 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Rat IL 21 General Information | |
|---|---|
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| Alternate Names | |
| Interleukin-21, IL-21, Il21. | |
| Molecular Weight | |
| 15.2 kDa | |
| Chromosomal Location | |
| q25 on chromosome 2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Il21 |
| Entrez Gene ID | 365769 |
| UniProt ID(s) | A3QPB9 |
| COSMIC ID Link(s) | Il21 |
| KEGG Gene ID(s) | rno:365769 |
| General Description of Recombinant Rat IL 21. | |
| Rat Interleukin-21 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 129 amino acids and having a total molecular mass of 15.2kDa. The Rat IL-21 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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