PDF DatasheetMouse IL 28A Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant IL 28A based upon sequence from Mouse
Host: QP10708 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of IL 28A was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized IL-28A in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The ED50 as determined by an anti-viral assay using human HepG2 cells infected with encephalomyocarditis(EMC) virus is less than 3 ng/ml, corresponding to a specific activity of > 3.3 × 105 IU/mg.
Amino Acid Sequence: DPVPRATRLP VEAKDCHIAQ FKSLSPKELQ AFKKAKDAIE KRLLEKDMRC SSHLISRAWD LKQLQVQERP KALQAEVALT LKVWENMTDS ALATILGQPL HTLSHIHSQL QTCTQLQATA EPKPPSRRLS RWLHRLQEAQ SKETPGCLED SVTSNLFRLL TRDLKCVASG DQCV
Purity: Greater than 95% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The Mouse IL-28A is lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4 and 5% trehalose.
Storage Conditions: Lyophilized IFN-Lambda 2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-Lambda 2 Recombinant should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Mouse IL 28A General Information | |
|---|---|
| Alternate Names | |
| Interferon lambda-2, IFN-lambda-2, Interleukin-28A, IL-28A, Ifnl2, Il28a, EG330496. | |
| Molecular Weight | |
| 19.7 kDa | |
| Chromosomal Location | |
| B1 on chromosome 7 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Ifnl2 |
| Entrez Gene ID | 330496 |
| UniProt ID(s) | Q4VK74 |
| COSMIC ID Link(s) | Ifnl2 |
| KEGG Gene ID(s) | mmu:330496 |
| General Description of Recombinant Mouse IL 28A. | |
| Recombinant Mouse Interleukin-28A produced in E. Coli is a single, non-glycosylated polypeptide chain containing 174 amino acids and having a total molecular mass of 19.7kDa. The IL-28A is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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