PDF DatasheetHuman IL 28B Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant IL 28B based upon sequence from Human
Host: QP10709 protein expressed in HEK 293.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of IL 28B was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized IL-28B in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The specific activity was determined by the dose dependent protection of the cytopathic effect on A549 cells (human lung adenocarcinoma epithelial cell line) that were challenged with encephalomyocarditis (EMC) virus and is typically 0.1-1ng/ml.
Amino Acid Sequence: MTGDCMPVLV LMAAVLTVTG AVPVARLRGA LPDARGCHIA QFKSLSPQEL QAFKRAKDAL EESLLLKDCK CRSRLFPRTW DLRQLQVRER PVALEAELAL TLKVLEATAD TDPALGDVLD QPLHTLHHIL SQLRACIQPQ PTAGPRTRGR LHHWLHRLQE APKKESPGCL EASVTFNLFR LLTRDLNCVA SGDLCV
Purity: Greater than 95% as obsereved by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The IL28B was lyophilized from 1 mg/ml in 1xPBS.
Storage Conditions: Lyophilized IL-28B although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL28B should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human IL 28B General Information | |
|---|---|
| Alternate Names | |
| Interleukin-28B, IL-28B, Cytokine Zcyto22, Interferon lambda-3, IFN-lambda-3, Interleukin-28C, IL-28C, IL28B, IFNL3, IL28C, ZCYTO22. | |
| Molecular Weight | |
| 24 kDa | |
| Chromosomal Location | |
| q13.2 on chromosome 19 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | IFNL3 |
| Entrez Gene ID | 282617 |
| UniProt ID(s) | Q8IZI9 |
| COSMIC ID Link(s) | IFNL3 |
| KEGG Gene ID(s) | hsa:282617 |
| General Description of Recombinant Human IL 28B. | |
| Human Interleukin-28B produced in HEK cells is a non-glycosylated monomer, having a total molecular weight of 24kDa. The IL28B is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bios products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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