PDF DatasheetHuman IL34 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant IL34 based upon sequence from Human
Host: QP10728 protein expressed in HEK 293.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of IL34 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Interleukin-34 in sterile 18M-cm H2O containing 0.1% endotoxin-free recombinant HSA.
Bioactivity Data: The specific activity was measured by its ability to induce secretion of MCP-1 from PBMCs.
Monomer or Dimer: Dimer
Amino Acid Sequence: NEPLEMWPLT QNEECTVTGF LRDKLQYRSR LQYMKHYFPI NYKISVPYEG VFRIANVTRL QRAQVSEREL RYLWVLVSLS ATESVQDVLL EGHPSWKYLQ EVETLLLNVQ QGLTDVEVSP QGLTDVEVSP APGPNLKLVR PKALLDNCFR VMELLYCSCC KQSSVLNWQD CEVPSPQSCS PEPSLQYAAT QLYPPPPWSP SSPPHSTGSV RPVRAQGEGL LPHHHHHHHH
Purity: Greater than 95.0% as determined by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The IL34 was lyophilized from a solution containing 20mM Sodium Citrate, pH 3.0 and 75mM NaCl.
Storage Conditions: Lyophilized IL34 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Interleukin 34 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human IL34 General Information | |
|---|---|
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| Alternate Names | |
| Interleukin 34, C16orf77, MGC34647, IL34. | |
| Molecular Weight | |
| 52.5 kDa | |
| Chromosomal Location | |
| q22.1 on chromosome 16 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | IL34 |
| Entrez Gene ID | 146433 |
| UniProt ID(s) | Q6ZMJ4 |
| COSMIC ID Link(s) | IL34 |
| KEGG Gene ID(s) | hsa:146433 |
| General Description of Recombinant Human IL34. | |
| Human Interleukin 34 produced in HEK cells is a glycosylated homodimer, containing 460 amino acids and having a total molecular weight of 52.5kDa. The IL34 protein is fused to a His-tag at C-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bios products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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