PDF DatasheetMouse IL36G Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant IL36G based upon sequence from Mouse
Host: QP10729 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of IL36G was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized IL36g in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The ED50 as determined by inducing IL-6 secretion in murine NIH/3T3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0×105 IU/mg.
Amino Acid Sequence: GRETPDFGEV FDLDQQVWIF RNQALVTVPR SHRVTPVSVT ILPCKYPESL EQDKGIAIYL GIQNPDKCLF CKEVNGHPTL LLKEEKILDL YHHPEPMKPF LFYHTRTGGT STFESVAFPG HYIASSKTGN PIFLTSKKGE YYNINFNLDI KS
Purity: Greater than 97.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: Lyophilized from a 0.2µm filtered concentrated solution in 20mM Tris-HCl, pH 8.5, 150mM NaCl and Tween-20.
Storage Conditions: Lyophilized IL36g Mouse although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL36g should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Mouse IL36G General Information | |
|---|---|
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| Alternate Names | |
| Interleukin-36 gamma, Interleukin-1 family member 9, IL-1F9, Il36g, Il1f9. | |
| Molecular Weight | |
| 17.3 kDa | |
| Chromosomal Location | |
| A3 on chromosome 2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Il36g |
| Entrez Gene ID | 215257 |
| UniProt ID(s) | Q8R460 |
| COSMIC ID Link(s) | Il36g |
| KEGG Gene ID(s) | mmu:215257 |
| General Description of Recombinant Mouse IL36G. | |
| Mouse IL36G produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 152 amino acids and having a molecular mass of 17.3kDa. The IL36G is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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