PDF DatasheetHuman Insulin Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Insulin based upon sequence from Human
Host: QP10732 protein expressed in S. cerevisiae, E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Insulin was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Insulin in PBS pH 7.5.
Bioactivity Data: 27.5 units/mg. Insulin has been evaluated in cell culture (Human Foreskin Fibroblasts). The effective concentration range for use in defined media is 1-5ug/ml. However, the optimum concentration range for a particular application must be determined by the investigator.
Amino Acid Sequence: MALWMRLLPL LALLALWGPD PAAAFVNQHL CGSHLVEALY LVCGERGFFY TPKTRREAED LQVGQVELGG GPGAGSLQPL ALEGSLQKRG IVEQCCTSIC SLYQLENYCN
Purity: Greater than 95.0% as determined by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The protein was lyophilized from 50mM Sodium Chloride solution.
Storage Conditions: Lyophilized Insulin although stable at room temperature, should be stored at 4°C. Upon reconstitution Insulin should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human Insulin General Information | |
|---|---|
 |
|
| Molecular Weight | |
| 5.8 kDa | |
| Chromosomal Location | |
| p15.5 on chromosome 11 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | INS |
| Entrez Gene ID | 3630 |
| UniProt ID(s) | P01308 |
| COSMIC ID Link(s) | INS |
| KEGG Gene ID(s) | hsa:3630 |
| General Description of Recombinant Human Insulin. | |
| Human Insulin produced in Yeast is a two chain, glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 5807 Dalton. Zinc content was found to be 0.4%. Insulin is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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