PDF DatasheetMouse KGF Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant KGF based upon sequence from Mouse
Host: QP10734 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of KGF was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized KGF in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The ED50, calculated by the dose-dependant stimulation of KGF-responsive BaF3 indicator cells (measured by 3H-thymidine uptake) is < 10ng/ml corresponding to a specific activity of 100,000 Units/mg.
Amino Acid Sequence: MCNDMSPEQT ATSVNCSSPE RHTRSYDYME GGDIRVRRLF CRTQWYLRID KRGKVKGTQE MKNSYNIMEI RTVAVGIVAI KGVESEYYLA MNKEGKLYAK KECNEDCNFK ELILENHYNT YASAKWTHSG GEMFVALNQK GIPVKGKKTK KEQKTAHFLP MAIT
Purity: Greater than 95.0% as determined by: (a) Analysis by HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The protein was lyophilized from a concentrated (1 mg/ml) solution containing 20mM Phosphate buffer pH 8 and 0.1M NaCl.
Storage Conditions: Lyophilized KGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF7 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Mouse KGF General Information | |
|---|---|
| Alternate Names | |
| HBGF-7, FGF7, FGF-7, KGF, Keratinocyte growth factor, Fibroblast growth factor 7, Heparin-binding growth factor 7. | |
| Molecular Weight | |
| 18.9 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P36363 |
| General Description of Recombinant Mouse KGF. | |
| Mouse Keratinocyte Growth Factor-1 produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 164 amino acids and having a molecular mass of 18.9 kDa. The FGF-7 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used for any other purpose. This product is guaranteed to work for a period of one year when stored at -70°C or colder.
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