PDF DatasheetL-Asparaginase Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant L-Asparaginase based upon sequence from other
Host: QP10740 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of L-Asparaginase was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Definition of Unit (for specific activity): One unit of enzyme catalyzes hydrolyzation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade.
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized L-Asparaginase in 18M-cm H2O at 1 mg/ml.
Bioactivity Data: One IU of L- Asparaginase is defined as that amount of enzyme required to generate 1 µmol of ammonia per minute at pH 7.3 and 37°C.
Specific Activity: 225 IU/mg.
Purity: Greater than 96.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The enzyme was lyophilized with no additives.
Storage Conditions: Lyophilized L-Asparaginase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution L-Asparaginase should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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