PDF DatasheetHuman LBP Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant LBP based upon sequence from Human
Host: QP10741 protein expressed in CHO.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of LBP was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: Every 10µg of recombinant human LBP should be reconstituted using 33µl of sterile H2O. The solution can be than diluted with phosphate-buffered saline or other buffers.
Bioactivity Data: Up to 0.2 µg/ ml LBP mediates binding of FITC-LPS (0.5µg/ml) to CD14+CHO transfectants (FACS).
Amino Acid Sequence: MGALARALPS ILLALLLTST PEALGANPGL VARITDKGLQ YAAQEGLLAL QSELLRITLP DFTGDLRIPH VGRGRYEFHS LNIHSCELLH SALRPVPGQG LSLSISDSSI RVQGRWKVRK SFFKLQGSFD VSVKGISISV NLLLGSESSG RPTVTASSCS SDIADVEVDM SGDLGWLLNL FHNQIESKFQ KVLESRICEM IQKSVSSDLQ PYLQTLPVTT EIDSFADIDY SLVEAPRATA QMLEVMFKGE IFHRNHRSPV TLLAAVMSLP EEHNKMVYFA ISDYVFNTAS LVYHEEGYLN FSITDDMIPP DSNIRLTTKS FRPFVPRLAR LYPNMNLELQ GSVPSAPLLN FSPGNLSVDP YMEIDAFVLL PSSSKEPVFR LSVATNVSAT LTFNTSKITG FLKPGKVKVE LKESKVGLFN AELLEALLNY YILNTFYPKF NDKLAEGFPL PLLKRVQLYD LGLQIHKDFL FLGANVQYMR V
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: Recombinant Human LBP was lyophilized from a protein solution (0.3 mg/ml) containing phosphate-buffered saline, pH 7.2.
Storage Conditions: Lyophilized LBP Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LBP should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human LBP General Information | |
|---|---|
| Alternate Names | |
| Lipopolysaccharide-binding protein, LBP, MGC22233. | |
| Molecular Weight | |
| 53.4 kDa | |
| Chromosomal Location | |
| q11.23 on chromosome 20 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | LBP |
| Entrez Gene ID | 3929 |
| UniProt ID(s) | P18428 |
| COSMIC ID Link(s) | LBP |
| KEGG Gene ID(s) | hsa:3929 |
| General Description of Recombinant Human LBP. | |
| Human The Lipopolysaccharide Binding Protein is produced from human LBP transfected CHO-cells in serum free medium. Before transfection the complete human LBP-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. The LBP was purified by his-tag with metal affinity purification with Talon and controlled by SDS page. Showing a 58kDa band on SDS-PAGE. Attention: His-tag has no protease site and can not be split off. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
There are no reviews yet.