PDF DatasheetOvine Leptin Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Leptin based upon sequence from Ovine
Host: QP10755 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Leptin was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Leptin in sterile 0.02% NaHCO3 not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Biological active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
Amino Acid Sequence: VPIRKVQDDT KTLIKTIVTR INDISHTQSV SSKQRVTGLD FIPGLHPLLS LSKMDQTLAI YQQILASLPS RNVIQISNDL ENLRDLLHLL AASKSCPLPQ VRALESLESL GVVLEASLYS TEVVALSRLQ GSLQDMLRQL DLSPGC
Purity: Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Concentration of Ovine Leptin Protein: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.2 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC,using calibrated solution of Leptin Ovine as a Reference Standard.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.02% NaHCO3.
Storage Conditions: Lyophilized Leptin Ovine MTS tagged although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leptin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Ovine Leptin General Information | |
|---|---|
| Alternate Names | |
| OB Protein, Obesity Protein, OBS, Obesity factor. | |
| Molecular Weight | |
| 17.5 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q28603 |
| General Description of Recombinant Ovine Leptin. | |
| Leptin Ovine MTS tagged Recombinant produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 157 amino acids and having a molecular mass of 17.5 kDa. The Leptin is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. The membrane translocating sequence Tag is composed of 10 amino acids Val-Leu-Leu-Pro-Val-Leu-Leu-Ala-Ala-Pro located at the N-terminus. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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