PDF DatasheetMouse LGALS8 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant LGALS8 based upon sequence from Mouse
Host: QP10769 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of LGALS8 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Other Applications: 1. Mix equal volumes of human blood and Alsevers solution (pH 7.0). (Alsevers solution: NaCl 0.42g, Sodium citric acid 0.8g, Citric acid 0.055g, D-glucose 2.05g in DW100 ml). 2. Centrifuge at 15000rpm for 10 minutes and wash 4 times with PBS. 3. Dilute packed cells in a 0.5 mg/ml trypsin-EDTA solution to give 4% red cell suspension. 4. Incubate for 1 hour at 37°C and wash 4 times with PBS. 5. Dilute packed cells in PBS to give a 4% red cell suspension. 6. Load 50µl of 0.5%BSA-in-0.15M-NaCl solution and 25µl of 4%-Red-Cell-in-PBS in U shaped wells. 7. Add 25µl of serial diluted galectin protein in PBS to each well plate (Round bottom 96 well plate). 8. Incubate for 30 minutes at room temperature to observe visible agglutination.
Bioactivity Data: The ED50 for this effect is ≤ 2ug/ml. Measured by its ability to agglutinate human red blood cells.
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMLSLNNL QNIIYNPIIP YVGTITEQLK PGSLIVIRGH VPKDSERFQV DFQLGNSLKP RADVAFHFNP RFKRSSCIVC NTLTQEKWGW EEITYDMPFR KEKSFEIVFM VLKNKFQVAV NGRHVLLYAH RISPEQIDTV GIYGKVNIHS IGFRFSSDLQSMETSALGLT QINRENIQKP GKLQLSLPFE ARLNASMGPG RTVVIKGEVN TNARSFNVDL VAGKTRDIAL HLNPRLNVKA FVRNSFLQDA WGEEERNITC FPFSSGMYFE MIIYCDVREF KVAINGVHSL EYKHRFKDLS SIDTLSVDGD IRLLDVRSW
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The LGALS8 solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Mouse LGALS8 General Information | |
|---|---|
| Alternate Names | |
| Galectin-8, Gal-8, LGALS-8, AI326142, D13Ertd524e, 1200015E08Rik. | |
| Molecular Weight | |
| 38 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q9JL15 |
| General Description of Recombinant Mouse LGALS8. | |
| Mouse LGALS8 produced E. coli is a single polypeptide chain containing 339 amino acids (1-316) and having a molecular mass of 38kDa. LGALS8 is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bios products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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