PDF Datasheetother Lipase A Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Lipase A based upon sequence from other
Host: QP10772 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Lipase A was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Definition of Unit (for specific activity): Lipase-A was assayed using p-nitrophenyl acetate ( pNPA) as a substrate. One unit of lipase activity was defined as the amount of enzyme releasing 1.0 ?mol of p-nitrophenol per minute.
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Lipase-A in sterile 10%-50% DMSO, isopropyl ether, petroleum ether, ethanol, acetone and isopropanol.
Additional Information: Stable from pH5~10. Unstable at higher than 50°C. Higher activity can be reached in 10%-50% DMSO, isopropyl ether and petroleum ether, 10%-25% ethanol, acetone and isopropanol, 50% activity can be kept in 50% acetone and isopropanol, no activity detected in 50% ethanol.
Bioactivity Data: 580 units/mg powder.
Purity: Greater than 90% as determined by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The protein was lyophilized without additives.
Storage Conditions: Recombinant Lipase-A although stable at room temp for 1 week, should be stored desiccated below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered lyophilized powder.
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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