PDF DatasheetMouse MCP 1 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant MCP 1 based upon sequence from Mouse
Host: QP10781 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of MCP 1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Monocyte Chemotactic Protein-1 in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The biological activity was determined by calculating its ability to chemoattract Balb/C mouse spleen MNCs at 1.0-20.0 ng/ml.
Amino Acid Sequence: MQVPVMLLGL LFTVAGWSIH VLAQPDAVNA PLTCCYSFTS KMIPMSRLES YKRITSSRCP KEAVVFVTKL KREVCADPKK EWVQTYIKNL DRNQMRSEPT TLFKTASALR SSAPLNVKLT RKSEANASTT FSTTTSSTSV GVTSVTVN
Purity: Greater than 90.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The protein was lyophilized with no additives.
Storage Conditions: Lyophilized MCP-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL2 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Mouse MCP 1 General Information | |
|---|---|
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| Alternate Names | |
| Small inducible cytokine A2, CCL2, Monocyte chemotactic protein 1, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, MCAF, Monocyte secretory protein JE, HC11, chemokine (C-C motif) ligand 2, MCP1, SCYA2, GDCF-2, SMC-CF, HSMCR30, MGC9434, GDCF-2 HC11, Platelet-derived growth factor-inducible protein JE. | |
| Molecular Weight | |
| 14 kDa | |
| Chromosomal Location | |
| C on chromosome 11 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Ccl2 |
| Entrez Gene ID | 20296 |
| UniProt ID(s) | P10148 |
| COSMIC ID Link(s) | Ccl2 |
| KEGG Gene ID(s) | mmu:20296 |
| General Description of Recombinant Mouse MCP 1. | |
| Mouse Monocyte Chemotactic Protein-1 produced in E. Coli is anon-glycosylated, polypeptide chain containing 125 amino acids and having a molecular mass of 14 kDa. The MCP-1 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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