Mouse Midkine Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Midkine based upon sequence from Mouse
Host: QP10786 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Midkine was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Midkine Mouse in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Fully biologically active when compared to standard. Determined by its ability to chemoattract human neutrophils using a concentration range of 10-100 ng/ml corresponding to a specific activity of 10,000-100,000IU/mg.
Amino Acid Sequence: VAKKKEKVKK GSECSEWTWG PCTPSSKDCG MGFREGTCGA QTQRVHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR QGTLKKARYN AQCQETIRVT KPCTSKTKSK TKAKKGKGKD
Purity: Greater than 95.0% as determined by HPLC and SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: Lyophilized from a 0.2um filtered concentrated solution in PBS, pH 7.4.
Storage Conditions: Lyophilized Midkine Mouse although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Midkine Mouse should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Recombinant Mouse Midkine General Information | |
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Alternate Names | |
NEGF-2, Neurite Growth-Promoting Factor 2, MK, Neurite outgrowth-promoting protein, Midgestation and kidney protein, Amphiregulin-associated protein, ARAP, Neurite outgrowth-promoting factor 2, FLJ27379, Midkine, MK1, NEGF2. | |
Molecular Weight | |
13.3 kDa | |
Curated Database and Bioinformatic Data | |
UniProt ID(s) | P12025 |
General Description of Recombinant Mouse Midkine. | |
Mouse Midkine produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 120 amino acids and having a molecular mass of 13.3kDa. The Midkine Mouse is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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